A 4-dimensional representation of antennal lobe output based on an ensemble of characterized projection neurons

Erich M. Staudacher*, Wolf Huetteroth, Joachim Schachtner, Kevin C. Daly

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A central problem facing studies of neural encoding in sensory systems is how to accurately quantify the extent of spatial and temporal responses. In this study, we take advantage of the relatively simple and stereotypic neural architecture found in invertebrates. We combine standard electrophysiological techniques, recently developed population analysis techniques, and novel anatomical methods to form an innovative 4-dimensional view of odor output representations in the antennal lobe of the moth Manduca sexta. This novel approach allows quantification of olfactory responses of characterized neurons with spike time resolution. Additionally, arbitrary integration windows can be used for comparisons with other methods such as imaging. By assigning statistical significance to changes in neuronal firing, this method can visualize activity across the entire antennal lobe. The resulting 4-dimensional representation of antennal lobe output complements imaging and multi-unit experiments yet provides a more comprehensive and accurate view of glomerular activation patterns in spike time resolution.

Original languageEnglish
Pages (from-to)208-223
Number of pages16
JournalJournal of Neuroscience Methods
Volume180
Issue number2
DOIs
Publication statusPublished - 15 Jun 2009
Externally publishedYes

Keywords

  • 3-Dimensional reconstruction
  • Ensemble analysis
  • Identified glomeruli
  • Intracellular recording and staining
  • Manduca sexta L.
  • Moth
  • Olfaction
  • Spike time resolution

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