A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate

Zhichao Zhang; Zefeng Li; Ruen Xie; Zhen Tian; Yicun Yao; Bingwu Yang; Jingao Zhang; Haili Ma; Lan Rao; Kuiru Wang; Binbin Yan; Xinzhu Sang; Chongxiu Yu; Bo Fu; Shengli Pu; Jinhui Yuan; Qiang Wu

doi:10.1016/j.talanta.2025.128384

A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate

Zhichao Zhang, Zefeng Li, Ruen Xie, Zhen Tian, Yicun Yao, Bingwu Yang, Jingao Zhang, Haili Ma, Lan Rao, Kuiru Wang, Binbin Yan, Xinzhu Sang, Chongxiu Yu, Bo Fu, Shengli Pu, Jinhui Yuan^*, Qiang Wu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05–5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.

Original language	English
Article number	128384
Pages (from-to)	1-9
Number of pages	9
Journal	Talanta
Volume	295
Early online date	25 May 2025
DOIs	https://doi.org/10.1016/j.talanta.2025.128384
Publication status	Published - 1 Dec 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

293T cell lysate detection
P53 protein
Seven-core fiber biosensor
Vernier effect

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10.1016/j.talanta.2025.128384

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@article{82fc984b8c0c46a3ba20988059bc1a98,

title = "A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate",

abstract = "P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05–5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.",

keywords = "293T cell lysate detection, P53 protein, Seven-core fiber biosensor, Vernier effect",

author = "Zhichao Zhang and Zefeng Li and Ruen Xie and Zhen Tian and Yicun Yao and Bingwu Yang and Jingao Zhang and Haili Ma and Lan Rao and Kuiru Wang and Binbin Yan and Xinzhu Sang and Chongxiu Yu and Bo Fu and Shengli Pu and Jinhui Yuan and Qiang Wu",

year = "2025",

month = dec,

day = "1",

doi = "10.1016/j.talanta.2025.128384",

language = "English",

volume = "295",

pages = "1--9",

journal = "Talanta",

issn = "0039-9140",

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}

TY - JOUR

T1 - A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate

AU - Zhang, Zhichao

AU - Li, Zefeng

AU - Xie, Ruen

AU - Tian, Zhen

AU - Yao, Yicun

AU - Yang, Bingwu

AU - Zhang, Jingao

AU - Ma, Haili

AU - Rao, Lan

AU - Wang, Kuiru

AU - Yan, Binbin

AU - Sang, Xinzhu

AU - Yu, Chongxiu

AU - Fu, Bo

AU - Pu, Shengli

AU - Yuan, Jinhui

AU - Wu, Qiang

PY - 2025/12/1

Y1 - 2025/12/1

N2 - P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05–5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.

AB - P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.05–5 ng/mL, and the limit of detection (LoD) can be as low as 0.0496 ng/mL. Moreover, the ability of the CTSCF biosensor to detect the p53 protein in 293T cell lysate was validated and compared with traditional western blotting (WB) method. The results show that the LoD of the CTSCF biosensor is much lower than that of the WB method. The proposed CTSCF biosensor has rapid detection time, good stability and specificity, high sensitivity, and low LoD, which has great potential for application in clinical medicine and pathophysiology.

KW - 293T cell lysate detection

KW - P53 protein

KW - Seven-core fiber biosensor

KW - Vernier effect

UR - https://www.scopus.com/pages/publications/105006474837

U2 - 10.1016/j.talanta.2025.128384

DO - 10.1016/j.talanta.2025.128384

M3 - Article

AN - SCOPUS:105006474837

SN - 0039-9140

VL - 295

SP - 1

EP - 9

JO - Talanta

JF - Talanta

M1 - 128384

ER -

A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate

Abstract

UN SDGs

Keywords

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