A Comprehensive Analysis of Replicative Lifespan in 4,698 Single-Gene Deletion Strains Uncovers Conserved Mechanisms of Aging

Mark A McCormick, Joe R Delaney, Mitsuhiro Tsuchiya, Scott Tsuchiyama, Anna Shemorry, Sylvia Sim, Annie Chia-Zong Chou, Umema Ahmed, Daniel Carr, Christopher J Murakami, Jennifer Schleit, George L Sutphin, Brian M Wasko, Christopher F Bennett, Adrienne M Wang, Brady Olsen, Richard P Beyer, Theodor K Bammler, Donna Prunkard, Simon C JohnsonJuniper K Pennypacker, Elroy An, Arieanna Anies, Anthony S Castanza, Eunice Choi, Nick Dang, Shiena Enerio, Marissa Fletcher, Lindsay Fox, Sarani Goswami, Sean A Higgins, Molly A Holmberg, Di Hu, Jessica Hui, Monika Jelic, Ki-Soo Jeong, Elijah Johnston, Emily O Kerr, Jin Kim, Diana Kim, Katie Kirkland, Shannon Klum, Soumya Kotireddy, Eric Liao, Michael Lim, Michael S Lin, Winston C Lo, Dan Lockshon, Hillary A Miller, Richard M Moller, Brian Muller, Jonathan Oakes, Diana N Pak, Zhao Jun Peng, Kim M Pham, Tom G Pollard, Prarthana Pradeep, Dillon Pruett, Dilreet Rai, Brett Robison, Ariana A Rodriguez, Bopharoth Ros, Michael Sage, Manpreet K Singh, Erica D Smith, Katie Snead, Amrita Solanky, Benjamin L Spector, Kristan K Steffen, Bie Nga Tchao, Marc K Ting, Helen Vander Wende, Dennis Wang, K Linnea Welton, Eric A Westman, Rachel B Brem, Xin-Guang Liu, Yousin Suh, Zhongjun Zhou, Matt Kaeberlein, Brian K Kennedy

Research output: Contribution to journalArticlepeer-review

176 Citations (Scopus)


Many genes that affect replicative lifespan (RLS) in the budding yeast Saccharomyces cerevisiae also affect aging in other organisms such as C. elegans and M. musculus. We performed a systematic analysis of yeast RLS in a set of 4,698 viable single-gene deletion strains. Multiple functional gene clusters were identified, and full genome-to-genome comparison demonstrated a significant conservation in longevity pathways between yeast and C. elegans. Among the mechanisms of aging identified, deletion of tRNA exporter LOS1 robustly extended lifespan. Dietary restriction (DR) and inhibition of mechanistic Target of Rapamycin (mTOR) exclude Los1 from the nucleus in a Rad53-dependent manner. Moreover, lifespan extension from deletion of LOS1 is nonadditive with DR or mTOR inhibition, and results in Gcn4 transcription factor activation. Thus, the DNA damage response and mTOR converge on Los1-mediated nuclear tRNA export to regulate Gcn4 activity and aging.

Original languageEnglish
Pages (from-to)895-906
Number of pages12
JournalCell Metabolism
Issue number5
Publication statusPublished - 3 Nov 2015
Externally publishedYes


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