A high-throughput screening method for determining the substrate scope of nitrilases

Gary Black; Nicola Brown; Justin Perry; P. David Randall; Graeme Turnbull; Meng Zhang

doi:10.1039/C4CC06021K

A high-throughput screening method for determining the substrate scope of nitrilases

Gary Black, Nicola Brown, Justin Perry, P. David Randall, Graeme Turnbull, Meng Zhang

Applied Sciences Department

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32 Citations (Scopus)

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Abstract

Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

Original language	English
Pages (from-to)	2660-2662
Journal	Chemical Communications
Volume	51
DOIs	https://doi.org/10.1039/C4CC06021K
Publication status	Published - 9 Jan 2015

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Screening method nitrilasesFinal published version, 249 KB

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title = "A high-throughput screening method for determining the substrate scope of nitrilases",

abstract = "Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.",

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AU - Black, Gary

AU - Brown, Nicola

AU - Perry, Justin

AU - Randall, P. David

AU - Turnbull, Graeme

AU - Zhang, Meng

PY - 2015/1/9

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N2 - Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

AB - Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

U2 - 10.1039/C4CC06021K

DO - 10.1039/C4CC06021K

M3 - Article

SN - 0022-4936

SN - 1359-7345

SN - 1364-548X

VL - 51

SP - 2660

EP - 2662

JO - Chemical Communications

JF - Chemical Communications

ER -

A high-throughput screening method for determining the substrate scope of nitrilases

Abstract

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