Abstract
The novelty of this study resides in the fabrication of a bio-sensing device, based on the surface acoustic wave (SAW) on a nanocrystalline ZnO film. The ZnO film was deposited using an rf magnetron sputtering at room temperature on silicon. The deposited films showed the c-axis-oriented crystallite with grain size of ∼40 nm. The immunosensing device was fabricated using photolithographic protocols on the film. As a model biomolecular recognition and immunosensing, biospecific interaction between a 6-(2,4-dinitrophenyl)aminohexanoic acid (DNP) antigen and its antibody was employed, demonstrating the shifts of resonant frequencies on SAW immunosensing device. The device exhibited a linearity as a function of the antibody concentration in the range of 20∼20,000 ng/ml.
Original language | English |
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Pages (from-to) | 7181-7185 |
Journal | Journal of Nanoscience and Nanotechnology |
Volume | 9 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2009 |
Keywords
- DNP
- immunosensing
- nanocrystalline ZNO
- SAW
- ZNO/SI