ABC medium, a new chromogenic agar for the selective isolation of Salmonella spp.

John Perry, Michael Ford, Jeffrey Taylor, Amanda Jones, Roger Freeman, Frances Kate Gould

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

We describe a new chromogenic agar medium, ABC medium (αβ-chromogenic medium), which includes two substrates, 3,4-cyclohexenoesculetin-β-D-galactoside and 5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside, to facilitate the selective isolation of Salmonella spp. This medium exploits the fact that Salmonella spp. may be distinguished from other members of the familyEnterobacteriaceae by the presence of α-galactosidase activity in the absence of β-galactosidase activity. A total of 1,022 strains of Salmonella spp. and 300 other gram-negative strains were inoculated onto this medium. Of these, 1,019 (99.7%) strains of Salmonella spp. produced a characteristic green colony, whereas only 1 strain (0.33%) of non-Salmonellaproduced a green colony. A total of 283 stool samples were cultured onto desoxycholate citrate (DC) agar and ABC medium by direct inoculation and after selective enrichment in selenite broth. Overall, the sensitivity and specificity were superior for ABC medium (100 and 90.5%, respectively) than for DC agar (88 and 26.9%, respectively). We conclude that ABC medium offers a high degree of specificity for the detection of Salmonella spp. in stool samples.
Original languageEnglish
Pages (from-to)766-768
JournalJournal of Clinical Microbiology
Volume37
Issue number3
Publication statusPublished - Mar 1999

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