Acitivity measurements as a tool to characterize the microbial composition of methanogenic environments

Jan Dolfing; Wim G.B.M. Bloeman

doi:10.1016/0167-7012(85)90002-8

Acitivity measurements as a tool to characterize the microbial composition of methanogenic environments

Jan Dolfing^*, Wim G.B.M. Bloeman

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

113 Citations (Scopus)

Abstract

A rapid and reliable method to assess the potential specific activity of methagenic sludge is presented. The method is based on the gas chromatographic analysis for methane in the headspace of closed vials. Gas is sampled with a pressure lock syringe, which allows quantification independent of the pressure prevailing in the vials. The influence of various parameters as substrates, pH, NaCl and NH₄ Cl concentrations on the activity of methaogenic sludge was investigated with this method. Data on the methanogenic activities on different substrate are discussed in terms of different physiological groups present in the sludge.

Original language	English
Pages (from-to)	1-12
Number of pages	12
Journal	Journal of Microbiological Methods
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/0167-7012(85)90002-8
Publication status	Published - Jun 1985
Externally published	Yes

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10.1016/0167-7012(85)90002-8

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title = "Acitivity measurements as a tool to characterize the microbial composition of methanogenic environments",

abstract = "A rapid and reliable method to assess the potential specific activity of methagenic sludge is presented. The method is based on the gas chromatographic analysis for methane in the headspace of closed vials. Gas is sampled with a pressure lock syringe, which allows quantification independent of the pressure prevailing in the vials. The influence of various parameters as substrates, pH, NaCl and NH4 Cl concentrations on the activity of methaogenic sludge was investigated with this method. Data on the methanogenic activities on different substrate are discussed in terms of different physiological groups present in the sludge.",

author = "Jan Dolfing and Bloeman, \{Wim G.B.M.\}",

year = "1985",

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T1 - Acitivity measurements as a tool to characterize the microbial composition of methanogenic environments

AU - Dolfing, Jan

AU - Bloeman, Wim G.B.M.

PY - 1985/6

Y1 - 1985/6

N2 - A rapid and reliable method to assess the potential specific activity of methagenic sludge is presented. The method is based on the gas chromatographic analysis for methane in the headspace of closed vials. Gas is sampled with a pressure lock syringe, which allows quantification independent of the pressure prevailing in the vials. The influence of various parameters as substrates, pH, NaCl and NH4 Cl concentrations on the activity of methaogenic sludge was investigated with this method. Data on the methanogenic activities on different substrate are discussed in terms of different physiological groups present in the sludge.

AB - A rapid and reliable method to assess the potential specific activity of methagenic sludge is presented. The method is based on the gas chromatographic analysis for methane in the headspace of closed vials. Gas is sampled with a pressure lock syringe, which allows quantification independent of the pressure prevailing in the vials. The influence of various parameters as substrates, pH, NaCl and NH4 Cl concentrations on the activity of methaogenic sludge was investigated with this method. Data on the methanogenic activities on different substrate are discussed in terms of different physiological groups present in the sludge.

UR - https://www.scopus.com/pages/publications/0021881443

U2 - 10.1016/0167-7012(85)90002-8

DO - 10.1016/0167-7012(85)90002-8

M3 - Article

AN - SCOPUS:0021881443

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SP - 1

EP - 12

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Acitivity measurements as a tool to characterize the microbial composition of methanogenic environments

Abstract

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