Abstract
This study examined whether intensity of endurance stimulus within a concurrent training paradigm influenced the phosphorylation of signaling proteins associated with the mTOR and AMPK networks. Eight male cyclists completed (1) resistance exercise (RES), 6 × 8 squats at 80% 1-RM; (2) resistance exercise and moderate intensity cycling of 40 min at 65% V̇O2peak, (RES + MIC); (3) resistance exercise and high intensity interval cycling of 40 min with 6 alternating 3 min intervals of 85 and 45% V̇O2peak (RES + HIIC), in a cross-over design. Muscle biopsies were collected at rest and 3 h post-RES. There was a main effect of condition for mTORS2448 (p = 0.043), with a greater response in the RES + MIC relative to RES condition (p = 0.033). There was a main effect of condition for AMPKα2T172 (p = 0.041), with a greater response in RES + MIC, relative to both RES + HIIC (p = 0.026) and RES (p = 0.046). There were no other condition effects for the remaining protein kinases assessed (p > 0.05). These data do not support a molecular interference effect in cyclists under controlled conditions. There was no intensity-dependent regulation of AMPK, nor differential activation of anabolism with the manipulation of endurance exercise intensity.
Original language | English |
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Article number | 10785 |
Number of pages | 11 |
Journal | Scientific Reports |
Volume | 11 |
Issue number | 1 |
Early online date | 24 May 2021 |
DOIs | |
Publication status | Published - Dec 2021 |
Keywords
- AMP-Activated Protein Kinases/metabolism
- Adult
- Cross-Over Studies
- Endurance Training/methods
- Exercise/physiology
- Humans
- Male
- Muscle, Skeletal/metabolism
- Phosphorylation
- Resistance Training/methods
- Signal Transduction
- TOR Serine-Threonine Kinases/metabolism