Aerobic exercise intensity does not affect the anabolic signaling following resistance exercise in endurance athletes

Thomas Jones*, Lee Eddens, Joe Kupusarevic, Davina Camargo Madeira Simoes, Matthew J. W. Fuber, K. A. Van Someren, Glyn Howatson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This study examined whether intensity of endurance stimulus within a concurrent training paradigm influenced the phosphorylation of signaling proteins associated with the mTOR and AMPK networks. Eight male cyclists completed (1) resistance exercise (RES), 6 × 8 squats at 80% 1-RM; (2) resistance exercise and moderate intensity cycling of 40 min at 65% V̇O2peak, (RES + MIC); (3) resistance exercise and high intensity interval cycling of 40 min with 6 alternating 3 min intervals of 85 and 45% V̇O2peak (RES + HIIC), in a cross-over design. Muscle biopsies were collected at rest and 3 h post-RES. There was a main effect of condition for mTORS2448 (p = 0.043), with a greater response in the RES + MIC relative to RES condition (p = 0.033). There was a main effect of condition for AMPKα2T172 (p = 0.041), with a greater response in RES + MIC, relative to both RES + HIIC (p = 0.026) and RES (p = 0.046). There were no other condition effects for the remaining protein kinases assessed (p > 0.05). These data do not support a molecular interference effect in cyclists under controlled conditions. There was no intensity-dependent regulation of AMPK, nor differential activation of anabolism with the manipulation of endurance exercise intensity.
Original languageEnglish
Article number10785
Number of pages11
JournalScientific Reports
Volume11
Issue number1
Early online date24 May 2021
DOIs
Publication statusE-pub ahead of print - 24 May 2021

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