The current socio‐economic scenarios have generated several challenges for any organization. Regional authorities have designed policies that combine supply–demand needs and innovative entrepreneurship programs. The alignment between regional and business strategies has become critical to ensure the necessary resources, skills and capabilities in the region. This article analyses the alignment of regional strategies (entrepreneurial innovation ecosystems) and business strategies (development of new entrepreneurial innovations). By adopting mixed theoretical approaches, we proposed a conceptual model to understand the role of institutional strategies on the definition of business strategies. Given the nature of this study, our methodological design combines a case study approach and an action research approach. Our results provide insights into the positive outcomes generated when regional strategies and business strategies are aligned.