An adjuvant-modulated vaccine response in human whole blood

Jalil Hakimi, Ali Azizi, Salvador Ausar, Stephen Todryk, Nausheen Rahman, Roger Brookes

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
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Abstract

The restimulation of an immune memory response by in vitro culture of blood cells with a specific antigen has been used as a way to gauge immunity to vaccines for decades. In this commentary we discuss a less appreciated application to support vaccine process development. We report that human whole blood from pre-primed subjects can generate a profound adjuvant-modulated, antigen-specific response to several different vaccine formulations. The response is able to differentiate subtle changes in the quality of an immune memory response to vaccine formulations and can be used to select optimal conditions relating to a particular manufacture process step. Whilst questions relating to closeness to in vivo vaccination remain, the approach is another big step nearer to the more relevant human response. It has special importance for new adjuvant development, complementing other preclinical in vivo and in vitro approaches to considerably de-risk progression of novel vaccines prior to and throughout early clinical development. Broader implications of the approach are discussed.
Original languageEnglish
Pages (from-to)2130-2134
JournalHuman Vaccines & Immunotherapeutics
Volume13
Issue number9
Early online date12 Jun 2017
DOIs
Publication statusE-pub ahead of print - 12 Jun 2017

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