An enhanced tilted-angle acoustic tweezer for mechanical phenotyping of cancer cells

Hanlin Wang, Joe Boardman, Xiaoyan Zhang, Chao Sun, Meng Cai, Jun Wei, zhiqiang Dong, Mingqian Feng, Dongfang Liang, Sheng Hu, Yu Qian, Shuang Dong, Yongqing Fu, Hamdi Torun, Aled Clayton, Zhenlin Wu, Zhihua Xie, Xin Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
25 Downloads (Pure)

Abstract

Acoustofluidic devices becomes one of the emerging and versatile tools for many biomedical applications. Most of the previous acoustofluidic devices are used for cells manipulation, and the few devices for cell phenotyping with a limitation in throughput. In this study, an enhanced tilted-angle (ETA) acoustofluidic device is developed and applied for mechanophenotyping of live cells. The ETA Device consists of an interdigital transducer which is positioned along a microfluidic channel. An inclination angle of 5° is introduced between the interdigital transducer and the liquid flow direction. The pressure nodes formed inside the acoustofluidic field in the channel deflect the biological cells from their original course in accordance with their mechanical properties, including volume, compressibility, and density. The threshold power for fully converging the cells to the pressure node is used to calculate the acoustic contrast factor. To demonstrate the ETA device in cell mechanophenotyping, and distinguishing between different cell types, further experimentation is carried out by using A549 (lung cancer cells), MDB-MA-231 (breast cancer cells), and leukocytes. The resulting acoustic contrast factors for the lung and breast cancer cells are different from that of the leukocytes by 27.9% and 21.5%, respectively. These results suggest this methodology can successfully distinguish and phenotype different cell types based on the acoustic contrast factor.
Original languageEnglish
Article number341120
Number of pages9
JournalAnalytica Chimica Acta
Volume1255
Early online date23 Mar 2023
DOIs
Publication statusPublished - 15 May 2023

Cite this