An invertron-like linear plasmid mediates intracellular survival and virulence in bovine isolates of Rhodococcus equi

Ana Valero-Rello, Alexia Hapeshi, Elisa Anastasi, Sonsiray Alvarez, Mariela Scortti, Wim G. Meijer, Iain MacArthur, José A. Vázquez-Boland

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

We report a novel host-associated virulence plasmid in Rhodococcus equi, pVAPN, carried by bovine isolates of this facultative intracellular pathogenic actinomycete. Surprisingly, pVAPN is a 120-kb invertron-like linear replicon unrelated to the circular virulence plasmids associated with equine (pVAPA) and porcine (pVAPB variant) R. equi isolates. pVAPN is similar to the linear plasmid pNSL1 from Rhodococcus sp. NS1 and harbors six new vap multigene family members (vapN to vapS) in a vap pathogenicity locus presumably acquired via en bloc mobilization from a direct predecessor of equine pVAPA. Loss of pVAPN rendered R. equi avirulent in macrophages and mice. Mating experiments using an in vivo transconjugant selection strategy demonstrated that pVAPN transfer is sufficient to confer virulence to a plasmid-cured R. equi recipient. Phylogenetic analyses assigned the vap multigene family complement from pVAPN, pVAPA, and pVAPB to seven monophyletic clades, each containing plasmid type-specific allelic variants of a precursor vap gene carried by the nearest vap island ancestor. Deletion of vapN, the predicted “bovine-type” allelic counterpart of vapA, essential for virulence in pVAPA, abrogated pVAPN-mediated intramacrophage proliferation and virulence in mice. Our findings support a model in which R. equi virulence is conferred by host-adapted plasmids. Their central role is mediating intracellular proliferation in macrophages, promoted by a key vap determinant present in the common ancestor of the plasmid-specific vap islands, with host tropism as a secondary trait selected during coevolution with specific animal species.
Original languageEnglish
Pages (from-to)2725-2737
Number of pages13
JournalInfection and Immunity
Volume83
Issue number7
Early online date15 May 2015
DOIs
Publication statusPublished - 1 Jul 2015
Externally publishedYes

Fingerprint

Dive into the research topics of 'An invertron-like linear plasmid mediates intracellular survival and virulence in bovine isolates of Rhodococcus equi'. Together they form a unique fingerprint.

Cite this