Analytical bias between species caused by matrix effects in quantitative analysis of a small-molecule pharmaceutical candidate in plasma

Nicholas Gray, Stuart McDougall, John Dean

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Background: Suppression or enhancement of MS ionization, particularly evident when electrospray is used as the source of ions, has been widely discussed. Methods: An assay for a small-molecule pharmaceutical in dog plasma between 1–300 ng/ml was validated with a mean bias across the calibration range of 5.0%. When the calibration sample matrix was substituted for human plasma, the mean bias across the range increased to 29.1%. A study of bias originating as a result of matrix effects, arising from endogenous glycerophosphocholine species, in plasma sources is discussed. Conclusion: A simple strategy to assess the potential of any unmitigated matrix effect to bias quantitative analysis by nonequivalent ionization induction or suppression is evaluated
Original languageEnglish
Pages (from-to)675-684
JournalBioanalysis
Volume4
Issue number6
DOIs
Publication statusPublished - 2012

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