Assessment of cell viability

Simon Johnson*, Vy Nguyen, David Coder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Cell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes or the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for determining the pre-fixation viability of fixed cells either before or after fixation with amine-reactive dyes suitable for a range of excitation wavelengths. Membrane-impermeable dead cell and live cell dyes as well as dye-exclusion procedures for microscopy are also included.

Original languageEnglish
Article number9.2
JournalCurrent Protocols in Cytometry
Volume64
Issue number1
DOIs
Publication statusPublished - 1 Apr 2013
Externally publishedYes

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