TY - JOUR
T1 - Associations between human bacterial pathogens and ARGs are magnified in leachates as landfill ages
AU - Wu, Dong
AU - Wang, Liuhong
AU - Su, Yinglong
AU - Dolfing, Jan
AU - Xie, Bing
N1 - Funding Information:
This work is supported by the Natural Science Foundation of China (21577038, 31370510) and Shanghai Tongji Gao-Tingyao Environ. Sci. Technol. Development Foundation (STGEF2017). The authors thank Mgr. H. Huang (LG Wastes Disposal Co. LTD) and Dr. Liu Hang for their supports in on-site landfill sampling and bioinformatics (Hong Kong Polytechnic University), respectively. Dong Wu would specifically like to thank The Society of Hong Kong Scholar for its supports in his postdoctoral research (XJ2018030).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/2/1
Y1 - 2021/2/1
N2 - Landfills constitute the largest treatment and disposal reservoirs of anthropogenic waste on earth and they are continuously releasing antibiotic resistance genes (ARGs) to the environment for decades via leachates. Little is known about the association between ARGs and human bacterial pathogens as a function of time. Here, we quantified 10 subtypes of ARGs, integrons, and human bacterial pathogens (HBPs). Except for the ARGs encoding resistance to sulfonamides, the subtypes encoding resistance to beta-lactams, macrolides, and aminoglycosides were not related to integrons (Spearman, P > 0.05). Over time presence of ARGs became increasingly more correlated with the presence of human bacterial pathogens (Procrustes test; R = 0.81, P < 0.05), which were primarily identified as the Proteobacteria, Actinobacteria, and Firmicutes. Rather than the prevalence of integrons, dynamics of the bacterial community, including the increased nitrogen metabolism activity of Proteobacteria and decreased bacterial diversity were assumed to lead to a magnified association between HBPs and target ARGs (Varpart; > 13%).
AB - Landfills constitute the largest treatment and disposal reservoirs of anthropogenic waste on earth and they are continuously releasing antibiotic resistance genes (ARGs) to the environment for decades via leachates. Little is known about the association between ARGs and human bacterial pathogens as a function of time. Here, we quantified 10 subtypes of ARGs, integrons, and human bacterial pathogens (HBPs). Except for the ARGs encoding resistance to sulfonamides, the subtypes encoding resistance to beta-lactams, macrolides, and aminoglycosides were not related to integrons (Spearman, P > 0.05). Over time presence of ARGs became increasingly more correlated with the presence of human bacterial pathogens (Procrustes test; R = 0.81, P < 0.05), which were primarily identified as the Proteobacteria, Actinobacteria, and Firmicutes. Rather than the prevalence of integrons, dynamics of the bacterial community, including the increased nitrogen metabolism activity of Proteobacteria and decreased bacterial diversity were assumed to lead to a magnified association between HBPs and target ARGs (Varpart; > 13%).
KW - Antibiotic resistance
KW - Human bacterial pathogens
KW - Landfills
KW - Nitrogen metabolism capacity
KW - Solid waste treatment
U2 - 10.1016/j.chemosphere.2020.128446
DO - 10.1016/j.chemosphere.2020.128446
M3 - Article
C2 - 33038756
AN - SCOPUS:85092095266
SN - 0045-6535
VL - 264
JO - Chemosphere
JF - Chemosphere
IS - Part 1
M1 - 128446
ER -
