TY - JOUR
T1 - Bacterial communities involved directly or indirectly in the anaerobic degradation of cellulose
AU - Bao, Yuanyuan
AU - Dolfing, Jan
AU - Wang, Baozhan
AU - Chen, Ruirui
AU - Huang, Miansong
AU - Li, Zhongpei
AU - Lin, Xiangui
AU - Feng, Youzhi
PY - 2019/4/1
Y1 - 2019/4/1
N2 - To determine bacterial communities involved, directly or indirectly, in the anaerobic degradation of cellulose, we conducted a microcosm experiment with soil treated with 13C-cellulose, 12C-cellulose, or without cellulose with analyses of DNA-based stable isotope probing (DNA-SIP), real-time quantitative PCR, and high-throughput sequencing. Firmicutes, Actinobacteria, Verrucomicrobia, and Fibrobacteres were the dominant bacterial phyla-degrading cellulose. Generally, bacteria possessing gene-encoding enzymes involved in the degradation of cellulose and hemicellulose were stimulated. Phylotypes affiliated to Geobacter were also stimulated by cellulose, probably due to their role in electron transfer. Nitrogen-fixing bacteria were also detected, probably due to the decreased N availability during cellulose degradation. High-throughput sequencing showed the presence of bacteria not incorporating 13C and probably involved in the priming effect caused by the addition of cellulose to soil. Collectively, our findings revealed that a more diverse microbial community than expected directly and indirectly participated in anaerobic cellulose degradation.
AB - To determine bacterial communities involved, directly or indirectly, in the anaerobic degradation of cellulose, we conducted a microcosm experiment with soil treated with 13C-cellulose, 12C-cellulose, or without cellulose with analyses of DNA-based stable isotope probing (DNA-SIP), real-time quantitative PCR, and high-throughput sequencing. Firmicutes, Actinobacteria, Verrucomicrobia, and Fibrobacteres were the dominant bacterial phyla-degrading cellulose. Generally, bacteria possessing gene-encoding enzymes involved in the degradation of cellulose and hemicellulose were stimulated. Phylotypes affiliated to Geobacter were also stimulated by cellulose, probably due to their role in electron transfer. Nitrogen-fixing bacteria were also detected, probably due to the decreased N availability during cellulose degradation. High-throughput sequencing showed the presence of bacteria not incorporating 13C and probably involved in the priming effect caused by the addition of cellulose to soil. Collectively, our findings revealed that a more diverse microbial community than expected directly and indirectly participated in anaerobic cellulose degradation.
KW - Cellulolytic bacteria
KW - Cellulose degradation
KW - Nitrogen-fixing bacteria
KW - Paddy soil
KW - Priming effect
KW - Syntrophic microorganisms
U2 - 10.1007/s00374-019-01342-1
DO - 10.1007/s00374-019-01342-1
M3 - Article
AN - SCOPUS:85060242827
VL - 55
SP - 201
EP - 211
JO - Biology and Fertility of Soils
JF - Biology and Fertility of Soils
SN - 0178-2762
IS - 3
ER -