TY - JOUR
T1 - Bacterial diversity in house dust
T2 - characterization of a core indoor microbiome
AU - Entwistle, Jane
AU - Thompson, Jonathan R.
AU - Argyraki, Ariadne
AU - Bashton, Matthew
AU - Bramwell, Lindsay
AU - Crown, Matthew
AU - Hursthouse, Andrew
AU - Jabeen, Khadija
AU - Marinho-Reis, Amélia P.
AU - Namdeo, Anil
AU - Nelson, Andrew
AU - Pearce, David
AU - Potgieter-Vermaak, Sanja
AU - Rasmussen, Pat E.
AU - Wragg, Joanna
N1 - Funding Information:
JE acknowledges funding from the Natural Environment Research Council (Research Grant NE/T004401/1). MB and MC acknowledge research funding from Research England’s Expanding Excellence in England (E3) Fund as part of the Hub for Biotechnology in the Built Environment. KJ acknowledges funding for her PhD research programme from Northumbria University. Open access publication fees were provide by Northumbria University.
Publisher Copyright:
Copyright © 2021 Thompson, Argyraki, Bashton, Bramwell, Crown, Hursthouse, Jabeen, Marinho Reis, Namdeo, Nelson, Pearce, Potgieter-Vermaak, Rasmussen, Wragg and Entwistle.
PY - 2021/11/23
Y1 - 2021/11/23
N2 - Our indoor microbiome consists of a wide range of microbial taxa. Whilst many of these microbes are benign, some are beneficial, some harmful, yet our knowledge of the spatial heterogeneity of bacterial assemblages in our residential environment remains limited. To investigate the existence of a common core house dust bacterial microbiome we selected household vacuum dusts, collected through a citizen science approach, from homes across two bioclimatic regions (UK, Oceanic/Maritime and Greece, Mediterranean). Following the extraction of DNA from each dust sample, we targeted the bacterial 16S rRNA gene using Illumina NextSeq sequencing. PERMANOVA analysis of the microbial communities at family level grouped samples within their distinct bioclimatic region and SIMPER analysis at genus level identified the statistically significant taxa responsible for driving diversity between these groups. A “common to all” core house dust microbiome consisted of Acinetobacter, Massalia, Rubellimicrobium, Sphingomonas and Staphylococcus; genera typically associated with human occupancy and common environmental sources. Additionally, a “unique location specific” microbiome was identified, reflective of the bioclimatic region. The Greek dusts indicated a lower average diversity than the UK house dusts, with a high abundance of Rhizobiaceae in the Greek samples. Our study highlights citizen science as a powerful approach to access the indoor residential environment, at scale, and establishes the existence of a “core” house dust microbiome independent of bioclimatic region.
AB - Our indoor microbiome consists of a wide range of microbial taxa. Whilst many of these microbes are benign, some are beneficial, some harmful, yet our knowledge of the spatial heterogeneity of bacterial assemblages in our residential environment remains limited. To investigate the existence of a common core house dust bacterial microbiome we selected household vacuum dusts, collected through a citizen science approach, from homes across two bioclimatic regions (UK, Oceanic/Maritime and Greece, Mediterranean). Following the extraction of DNA from each dust sample, we targeted the bacterial 16S rRNA gene using Illumina NextSeq sequencing. PERMANOVA analysis of the microbial communities at family level grouped samples within their distinct bioclimatic region and SIMPER analysis at genus level identified the statistically significant taxa responsible for driving diversity between these groups. A “common to all” core house dust microbiome consisted of Acinetobacter, Massalia, Rubellimicrobium, Sphingomonas and Staphylococcus; genera typically associated with human occupancy and common environmental sources. Additionally, a “unique location specific” microbiome was identified, reflective of the bioclimatic region. The Greek dusts indicated a lower average diversity than the UK house dusts, with a high abundance of Rhizobiaceae in the Greek samples. Our study highlights citizen science as a powerful approach to access the indoor residential environment, at scale, and establishes the existence of a “core” house dust microbiome independent of bioclimatic region.
KW - Vacuum dust
KW - Bacterial assemblage
KW - citizen science
KW - 16S
KW - microbiome
UR - http://www.scopus.com/inward/record.url?scp=85120887936&partnerID=8YFLogxK
U2 - 10.3389/fenvs.2021.754657
DO - 10.3389/fenvs.2021.754657
M3 - Article
AN - SCOPUS:85120887936
SN - 2296-665X
VL - 9
SP - 754657
JO - Frontiers in Environmental Science
JF - Frontiers in Environmental Science
M1 - 754657
ER -