TY - CHAP
T1 - Biocatalysis
T2 - Important considerations for testing and evaluation of biocatalysts
AU - Tischler, Dirk
AU - Barone, Giovanni Davide
AU - Munoz-Munoz, Jose
AU - Woodley, John M.
PY - 2025/2/25
Y1 - 2025/2/25
N2 - Selecting the appropriate mode of biocatalysis application is crucial for optimizing efficiency and sustainability. This chapter provides a comprehensive guide on key metrics to describe biocatalyst performance, including kinetic parameters such as reaction rates, cofactor requirements, dissociation constants (KD), maximum velocities (Vmax), turnover numbers (kcat), and Michaelis constants (KM). Additionally, it discusses biocatalysis metrics like turnover frequency (TOF), environmental factors (E-Factor), atom economy, productivities, and Life Cycle Assessment (LCA). The chapter also explores application types, focusing on whole-cell and cell-free enzyme applications, and offers a practical guide on selecting the most suitable mode of application based on specific project requirements. By integrating these considerations, researchers can effectively harness biocatalysis for innovative and sustainable solutions in various industrial processes.
AB - Selecting the appropriate mode of biocatalysis application is crucial for optimizing efficiency and sustainability. This chapter provides a comprehensive guide on key metrics to describe biocatalyst performance, including kinetic parameters such as reaction rates, cofactor requirements, dissociation constants (KD), maximum velocities (Vmax), turnover numbers (kcat), and Michaelis constants (KM). Additionally, it discusses biocatalysis metrics like turnover frequency (TOF), environmental factors (E-Factor), atom economy, productivities, and Life Cycle Assessment (LCA). The chapter also explores application types, focusing on whole-cell and cell-free enzyme applications, and offers a practical guide on selecting the most suitable mode of application based on specific project requirements. By integrating these considerations, researchers can effectively harness biocatalysis for innovative and sustainable solutions in various industrial processes.
KW - Biotransformation
KW - Enzyme catalysis
KW - Fermentation
KW - Immobilization
KW - Industrial biotechnology
KW - Key metrics
KW - Whole-cell catalysis
UR - http://www.scopus.com/inward/record.url?scp=85218871299&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2025.01.080
DO - 10.1016/bs.mie.2025.01.080
M3 - Chapter
AN - SCOPUS:85218871299
T3 - Methods in Enzymology
BT - Methods in Enzymology
PB - Academic Press Inc.
ER -
