Biocatalysis: Important considerations for testing and evaluation of biocatalysts

Dirk Tischler; Giovanni Davide Barone; Jose Munoz-Munoz; John M. Woodley

doi:10.1016/bs.mie.2025.01.080

Biocatalysis: Important considerations for testing and evaluation of biocatalysts

Dirk Tischler^*, Giovanni Davide Barone, Jose Munoz-Munoz, John M. Woodley

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Selecting the appropriate mode of biocatalysis application is crucial for optimizing efficiency and sustainability. This chapter provides a comprehensive guide on key metrics to describe biocatalyst performance, including kinetic parameters such as reaction rates, cofactor requirements, dissociation constants (K_D), maximum velocities (V_max), turnover numbers (k_cat), and Michaelis constants (K_M). Additionally, it discusses biocatalysis metrics like turnover frequency (TOF), environmental factors (E-Factor), atom economy, productivities, and Life Cycle Assessment (LCA). The chapter also explores application types, focusing on whole-cell and cell-free enzyme applications, and offers a practical guide on selecting the most suitable mode of application based on specific project requirements. By integrating these considerations, researchers can effectively harness biocatalysis for innovative and sustainable solutions in various industrial processes.

Original language	English
Title of host publication	Methods in Enzymology
Editors	Dirk Tischler
Place of Publication	Amsterdam, Netherlands
Publisher	Academic Press Inc.
Chapter	1
Pages	1-17
Number of pages	18
Volume	714
ISBN (Print)	9780443317880
DOIs	https://doi.org/10.1016/bs.mie.2025.01.080
Publication status	Published - 2025

Publication series

Name	Methods in Enzymology
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure
SDG 12 Responsible Consumption and Production

Keywords

Biotransformation
Enzyme catalysis
Fermentation
Immobilization
Industrial biotechnology
Key metrics
Whole-cell catalysis

Access to Document

10.1016/bs.mie.2025.01.080

Cite this

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title = "Biocatalysis: Important considerations for testing and evaluation of biocatalysts",

abstract = "Selecting the appropriate mode of biocatalysis application is crucial for optimizing efficiency and sustainability. This chapter provides a comprehensive guide on key metrics to describe biocatalyst performance, including kinetic parameters such as reaction rates, cofactor requirements, dissociation constants (KD), maximum velocities (Vmax), turnover numbers (kcat), and Michaelis constants (KM). Additionally, it discusses biocatalysis metrics like turnover frequency (TOF), environmental factors (E-Factor), atom economy, productivities, and Life Cycle Assessment (LCA). The chapter also explores application types, focusing on whole-cell and cell-free enzyme applications, and offers a practical guide on selecting the most suitable mode of application based on specific project requirements. By integrating these considerations, researchers can effectively harness biocatalysis for innovative and sustainable solutions in various industrial processes.",

keywords = "Biotransformation, Enzyme catalysis, Fermentation, Immobilization, Industrial biotechnology, Key metrics, Whole-cell catalysis",

author = "Dirk Tischler and Barone, \{Giovanni Davide\} and Jose Munoz-Munoz and Woodley, \{John M.\}",

year = "2025",

doi = "10.1016/bs.mie.2025.01.080",

language = "English",

isbn = "9780443317880",

volume = "714",

series = "Methods in Enzymology",

publisher = "Academic Press Inc.",

pages = "1--17",

editor = "Dirk Tischler",

booktitle = "Methods in Enzymology",

address = "United States",

}

Biocatalysis: Important considerations for testing and evaluation of biocatalysts. / Tischler, Dirk; Barone, Giovanni Davide; Munoz-Munoz, Jose et al.
Methods in Enzymology. ed. / Dirk Tischler. Vol. 714 Amsterdam, Netherlands : Academic Press Inc., 2025. p. 1-17 (Methods in Enzymology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Biocatalysis

T2 - Important considerations for testing and evaluation of biocatalysts

AU - Tischler, Dirk

AU - Barone, Giovanni Davide

AU - Munoz-Munoz, Jose

AU - Woodley, John M.

PY - 2025

Y1 - 2025

N2 - Selecting the appropriate mode of biocatalysis application is crucial for optimizing efficiency and sustainability. This chapter provides a comprehensive guide on key metrics to describe biocatalyst performance, including kinetic parameters such as reaction rates, cofactor requirements, dissociation constants (KD), maximum velocities (Vmax), turnover numbers (kcat), and Michaelis constants (KM). Additionally, it discusses biocatalysis metrics like turnover frequency (TOF), environmental factors (E-Factor), atom economy, productivities, and Life Cycle Assessment (LCA). The chapter also explores application types, focusing on whole-cell and cell-free enzyme applications, and offers a practical guide on selecting the most suitable mode of application based on specific project requirements. By integrating these considerations, researchers can effectively harness biocatalysis for innovative and sustainable solutions in various industrial processes.

AB - Selecting the appropriate mode of biocatalysis application is crucial for optimizing efficiency and sustainability. This chapter provides a comprehensive guide on key metrics to describe biocatalyst performance, including kinetic parameters such as reaction rates, cofactor requirements, dissociation constants (KD), maximum velocities (Vmax), turnover numbers (kcat), and Michaelis constants (KM). Additionally, it discusses biocatalysis metrics like turnover frequency (TOF), environmental factors (E-Factor), atom economy, productivities, and Life Cycle Assessment (LCA). The chapter also explores application types, focusing on whole-cell and cell-free enzyme applications, and offers a practical guide on selecting the most suitable mode of application based on specific project requirements. By integrating these considerations, researchers can effectively harness biocatalysis for innovative and sustainable solutions in various industrial processes.

KW - Biotransformation

KW - Enzyme catalysis

KW - Fermentation

KW - Immobilization

KW - Industrial biotechnology

KW - Key metrics

KW - Whole-cell catalysis

UR - https://www.scopus.com/pages/publications/85218871299

U2 - 10.1016/bs.mie.2025.01.080

DO - 10.1016/bs.mie.2025.01.080

M3 - Chapter

AN - SCOPUS:85218871299

SN - 9780443317880

VL - 714

T3 - Methods in Enzymology

SP - 1

EP - 17

BT - Methods in Enzymology

A2 - Tischler, Dirk

PB - Academic Press Inc.

CY - Amsterdam, Netherlands

ER -

Biocatalysis: Important considerations for testing and evaluation of biocatalysts

Abstract

Publication series

UN SDGs

Keywords

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