TY - JOUR
T1 - Biochemical analysis of cross‐feeding behaviour between two common gut commensals when cultivated on plant‐derived arabinogalactan
AU - Munoz, Jose
AU - James, Kieran
AU - Bottacini, Francesca
AU - Van Sinderen, Douwe
N1 - Funding Information:
J.M. received financial support from an internal grant in Northumbria University. D.v.S., F.B. and K.J. are members of APC Microbiome Ireland which received financial support from Science Foundation Ireland, through the Irish Government?s National Development Plan (SFI/12/RC/2273-P1 and SFI/12/RC/2273-P2). J.M. received financial support from an internal grant in Northumbria University. D.v.S., F.B. and K.J. are members of APC Microbiome Ireland which received financial support from Science Foundation Ireland, through the Irish Government?s National Development Plan (SFI/12/RC/2273-P1 and SFI/12/RC/2273-P2).
Publisher Copyright:
© 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - In this paper, we reveal and characterize cross‐feeding behaviour between the common gut commensal Bacteroides cellulosilyticus (Baccell) and certain bifidobacterial strains, including Bifidobacterium breve UCC2003, when grown on a medium containing Larch Wood Arabinogalactan (LW‐AG). We furthermore show that cross‐feeding is dependent on the release of β‐1,3‐galacto‐di/trisaccharides (β‐1,3‐GOS), and identified that the bga gene cluster of B. breve UCC2003 allows β‐1,3‐GOS metabolism. The product of bgaB is presumed to be responsible for the import of β‐1,3‐GOS, while the bgaA gene product, a glycoside hydrolase family 2 member, was shown to hydrolyse both β‐1,3‐galactobiose and β‐1,3‐galactotriose into galactose monomers. This study advances our understanding of strain‐specific syntrophic interactions between two glycan degraders in the human gut in the presence of AG‐type dietary polysaccharides.
AB - In this paper, we reveal and characterize cross‐feeding behaviour between the common gut commensal Bacteroides cellulosilyticus (Baccell) and certain bifidobacterial strains, including Bifidobacterium breve UCC2003, when grown on a medium containing Larch Wood Arabinogalactan (LW‐AG). We furthermore show that cross‐feeding is dependent on the release of β‐1,3‐galacto‐di/trisaccharides (β‐1,3‐GOS), and identified that the bga gene cluster of B. breve UCC2003 allows β‐1,3‐GOS metabolism. The product of bgaB is presumed to be responsible for the import of β‐1,3‐GOS, while the bgaA gene product, a glycoside hydrolase family 2 member, was shown to hydrolyse both β‐1,3‐galactobiose and β‐1,3‐galactotriose into galactose monomers. This study advances our understanding of strain‐specific syntrophic interactions between two glycan degraders in the human gut in the presence of AG‐type dietary polysaccharides.
UR - http://www.scopus.com/inward/record.url?scp=85084493087&partnerID=8YFLogxK
U2 - 10.1111/1751-7915.13577
DO - 10.1111/1751-7915.13577
M3 - Article
C2 - 32385941
SN - 1751-7915
VL - 13
SP - 1733
EP - 1747
JO - Microbial Biotechnology
JF - Microbial Biotechnology
IS - 6
ER -