Bioinformatic interrogation of expression array data to identify nutritionally regulated genes potentially modulated by DNA methylation

J. A. McKay*, M. E. Adriaens, F. D. Ford, C. L. Relton, C. T A Evelo, J. C. Mathers

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

DNA methylation occurs at CpG dinucleotide sites within the genome and is recognised as one of the mechanisms involved in regulation of gene expression. CpG sites are relatively underrepresented in the mammalian genome, but occur densely in regions called CpG islands (CGIs). CGIs located in the promoters of genes inhibit transcription when methylated by impeding transcription factor binding. Due to the malleable nature of DNA methylation, environmental factors are able to influence promoter CGI methylation patterns and thus influence gene expression. Recent studies have provided evidence that nutrition (and other environmental exposures) can cause altered CGI methylation but, with a few exceptions, the genes influenced by these exposures remain largely unknown. Here we describe a novel bioinformatics approach for the analysis of gene expression microarray data designed to identify regulatory sites within promoters of differentially expressed genes that may be influenced by changes in DNA methylation.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalGenes and Nutrition
Volume3
Early online date26 Nov 2008
DOIs
Publication statusPublished - 1 Dec 2008
Externally publishedYes

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