Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo

Jiawen Li; Yijian  Li; Xisu  Hu; Yu Gong; Ben Bin Xu; Haiwei Xu; Zheng Qin Yin

doi:10.18240/ijo.2020.10.03

Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo

Jiawen Li, Yijian Li, Xisu Hu, Yu Gong, Ben Bin Xu, Haiwei Xu^*, Zheng Qin Yin^*

^*Corresponding author for this work

Mechanical and Construction Engineering Department

Research output: Contribution to journal › Article › peer-review

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Abstract

The biosafety of the poly(acrylamide-co-sodium acrylate) hydrogel was first 19 analyzed in vitro using human lens epithelial cells (LECs) and the ARPE19 cell line, and a CCK-8 assay was performed to investigate alterations in cell proliferation. A thin film of a poly(acrylamide-co-sodium acrylate) hydrogel and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively, and a sham surgery served as control group. The anterior segment photographs, intraocular pressure (IOP), blood parameters and electroretinograms were recorded. Inflammatory cytokines in the aqueous humor, such as TNF and IL-8, were examined by ELISA. Cell apoptosis of the retina was investigated by TUNEL assay, and macrophage activation was detected by immunostaining.

Original language	English
Pages (from-to)	1521-1530
Journal	International Journal of Ophthalmology
Volume	13
Issue number	10
DOIs	https://doi.org/10.18240/ijo.2020.10.03
Publication status	Published - 18 Oct 2020

Keywords

Cataract
Biosafety
Lens Epithelial Cells
ARPE19 Cells
Intraocular Lens
3D 39 Printing
Poly Hydrogel
Rabbit

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title = "Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo",

abstract = "The biosafety of the poly(acrylamide-co-sodium acrylate) hydrogel was first 19 analyzed in vitro using human lens epithelial cells (LECs) and the ARPE19 cell line, and a CCK-8 assay was performed to investigate alterations in cell proliferation. A thin film of a poly(acrylamide-co-sodium acrylate) hydrogel and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively, and a sham surgery served as control group. The anterior segment photographs, intraocular pressure (IOP), blood parameters and electroretinograms were recorded. Inflammatory cytokines in the aqueous humor, such as TNF and IL-8, were examined by ELISA. Cell apoptosis of the retina was investigated by TUNEL assay, and macrophage activation was detected by immunostaining.",

keywords = "Cataract, Biosafety, Lens Epithelial Cells, ARPE19 Cells, Intraocular Lens, 3D 39 Printing, Poly Hydrogel, Rabbit",

author = "Jiawen Li and Yijian Li and Xisu Hu and Yu Gong and Xu, {Ben Bin} and Haiwei Xu and Yin, {Zheng Qin}",

year = "2020",

month = oct,

day = "18",

doi = "10.18240/ijo.2020.10.03",

language = "English",

volume = "13",

pages = "1521--1530",

journal = "International Journal of Ophthalmology",

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T1 - Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo

AU - Li, Jiawen

AU - Li, Yijian

AU - Hu, Xisu

AU - Gong, Yu

AU - Xu, Ben Bin

AU - Xu, Haiwei

AU - Yin, Zheng Qin

PY - 2020/10/18

Y1 - 2020/10/18

N2 - The biosafety of the poly(acrylamide-co-sodium acrylate) hydrogel was first 19 analyzed in vitro using human lens epithelial cells (LECs) and the ARPE19 cell line, and a CCK-8 assay was performed to investigate alterations in cell proliferation. A thin film of a poly(acrylamide-co-sodium acrylate) hydrogel and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively, and a sham surgery served as control group. The anterior segment photographs, intraocular pressure (IOP), blood parameters and electroretinograms were recorded. Inflammatory cytokines in the aqueous humor, such as TNF and IL-8, were examined by ELISA. Cell apoptosis of the retina was investigated by TUNEL assay, and macrophage activation was detected by immunostaining.

AB - The biosafety of the poly(acrylamide-co-sodium acrylate) hydrogel was first 19 analyzed in vitro using human lens epithelial cells (LECs) and the ARPE19 cell line, and a CCK-8 assay was performed to investigate alterations in cell proliferation. A thin film of a poly(acrylamide-co-sodium acrylate) hydrogel and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively, and a sham surgery served as control group. The anterior segment photographs, intraocular pressure (IOP), blood parameters and electroretinograms were recorded. Inflammatory cytokines in the aqueous humor, such as TNF and IL-8, were examined by ELISA. Cell apoptosis of the retina was investigated by TUNEL assay, and macrophage activation was detected by immunostaining.

KW - Cataract

KW - Biosafety

KW - Lens Epithelial Cells

KW - ARPE19 Cells

KW - Intraocular Lens

KW - 3D 39 Printing

KW - Poly Hydrogel

KW - Rabbit

U2 - 10.18240/ijo.2020.10.03

DO - 10.18240/ijo.2020.10.03

M3 - Article

C2 - 33078100

SN - 2222-3959

VL - 13

SP - 1521

EP - 1530

JO - International Journal of Ophthalmology

JF - International Journal of Ophthalmology

IS - 10

ER -

Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo

Abstract

Keywords

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