Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo

Research output: Contribution to journalArticle

Authors

  • Jiawen Li
  • Yijian Li
  • Xisu Hu
  • Yu Gong
  • Ben Bin Xu
  • Haiwei Xu
  • Zheng Qin Yin

External departments

  • Key Lab of Visual Damage and Regeneration & Restoration of Chongqing
  • Third Military Medical University

Details

Original languageEnglish
JournalInternational Journal of Ophthalmology
Publication statusAccepted/In press - 6 Aug 2020
Publication type

Research output: Contribution to journalArticle

Abstract

The biosafety of the poly(acrylamide-co-sodium acrylate) hydrogel was first 19 analyzed in vitro using human lens epithelial cells (LECs) and the ARPE19 cell line, and a CCK-8 assay was performed to investigate alterations in cell proliferation. A thin film of a poly(acrylamide-co-sodium acrylate) hydrogel and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively, and a sham surgery served as control group. The anterior segment photographs, intraocular pressure (IOP), blood parameters and electroretinograms were recorded. Inflammatory cytokines in the aqueous humor, such as TNF and IL-8, were examined by ELISA. Cell apoptosis of the retina was investigated by TUNEL assay, and macrophage activation was detected by immunostaining.