Biotransformation of nitriles using the solvent-tolerant nitrile hydratase from Rhodopseudomonas palustris CGA009

Gary Black; Thomas Gregson; Christopher McPake; Justin Perry; Meng Zhang

doi:10.1016/j.tetlet.2009.12.094

Biotransformation of nitriles using the solvent-tolerant nitrile hydratase from Rhodopseudomonas palustris CGA009

Gary Black, Thomas Gregson, Christopher McPake, Justin Perry, Meng Zhang

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A study has been carried out into the biocatalytic hydration of nitriles using the nitrile hydratase enzyme from Rhodopseudomonas palustris CGA009. It has been shown that this nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. The major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates.

Original language	English
Pages (from-to)	1639-1641
Journal	Tetrahedron Letters
Volume	51
Issue number	13
DOIs	https://doi.org/10.1016/j.tetlet.2009.12.094
Publication status	Published - Mar 2010

Keywords

Nitriles
Hydration
Organic solvents

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10.1016/j.tetlet.2009.12.094

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abstract = "A study has been carried out into the biocatalytic hydration of nitriles using the nitrile hydratase enzyme from Rhodopseudomonas palustris CGA009. It has been shown that this nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. The major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates.",

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AU - Black, Gary

AU - Gregson, Thomas

AU - McPake, Christopher

AU - Perry, Justin

AU - Zhang, Meng

PY - 2010/3

Y1 - 2010/3

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AB - A study has been carried out into the biocatalytic hydration of nitriles using the nitrile hydratase enzyme from Rhodopseudomonas palustris CGA009. It has been shown that this nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. The major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates.

KW - Nitriles

KW - Hydration

KW - Organic solvents

UR - https://www.scopus.com/pages/publications/76949102360

U2 - 10.1016/j.tetlet.2009.12.094

DO - 10.1016/j.tetlet.2009.12.094

M3 - Article

SN - 0040-4039

VL - 51

SP - 1639

EP - 1641

JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 13

ER -

Biotransformation of nitriles using the solvent-tolerant nitrile hydratase from Rhodopseudomonas palustris CGA009

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Keywords

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