Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells

Andrew D. Beale, Emily Kruchek, Stephen J. Kitcatt, Erin A. Henslee, Jack S.W. Parry, Gabriella Braun, Rita Jabr, Malcolm von Schantz, John S. O’Neill, Fatima H. Labeed*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Temperature compensation and period determination by casein kinase 1 (CK1) are conserved features of eukaryotic circadian rhythms, whereas the clock gene transcription factors that facilitate daily gene expression rhythms differ between phylogenetic kingdoms. Human red blood cells (RBCs) exhibit temperature-compensated circadian rhythms, which, because RBCs lack nuclei, must occur in the absence of a circadian transcription-translation feedback loop. We tested whether period determination and temperature compensation are dependent on CKs in RBCs. As with nucleated cell types, broad-spectrum kinase inhibition with staurosporine lengthened the period of the RBC clock at 37°C, with more specific inhibition of CK1 and CK2 also eliciting robust changes in circadian period. Strikingly, inhibition of CK1 abolished temperature compensation and increased the Q10 for the period of oscillation in RBCs, similar to observations in nucleated cells. This indicates that CK1 activity is essential for circadian rhythms irrespective of the presence or absence of clock gene expression cycles.

Original languageEnglish
Pages (from-to)144-153
Number of pages10
JournalJournal of Biological Rhythms
Volume34
Issue number2
Early online date21 Mar 2019
DOIs
Publication statusPublished - 1 Apr 2019
Externally publishedYes

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