Characterization of a truncated lipoarabinomannan from the actinomycete Turicella otitidis

Martine Gilleron, Natalie Garton, Jerome Nigou, Thérèse Brando, Germain Puzo, Iain Sutcliffe

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Lipoarabinomannan (LAM) lipoglycans have been characterized from a range of mycolic acid-containing actinomycetes and from the amycolate actinomycete Amycolatopsis sulphurea. To further understand the structural diversity of this family, we have characterized the lipoglycan of the otic commensal Turicella otitidis. T. otitidis LAM (TotLAM) lias been determined to consist of a mannosyl phosphatidylinositol anchor unit carrying an (α 1→6)-linked mannan core and substituted with terminal-arabinosyl branches. Thus, TotLAM has a novel truncated LAM structure. Using the human monocytic THP-1 cell line, it was found that TotLAM exhibited only minimal ability to induce tumor necrosis factor alpha. These findings contribute further to our understanding of actinomycete LAM diversity and allow further speculation as to the correlation between LAM structure and the immunomodulatory activities of these lipoglycans.
Original languageEnglish
Pages (from-to)854-861
JournalJournal of Bacteriology
Volume187
Issue number3
DOIs
Publication statusPublished - Feb 2005

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