Chromogenic enzyme substrates based on [2-(nitroaryl)ethenyl]pyridinium and quinolinium derivatives for the detection of nitroreductase activity in clinically important microorganisms

Marie Cellier-Rastit, Valérie Chalansonnet, Arthur L. James, Annette Johnston, Sylvain Orenga, John D. Perry, Celine Roger-Dalbert, Vindhya L. Salwatura, Steven Stanforth*, Hannah E. Sykes, Viet Thang Truong, Graeme Turnbull

*Corresponding author for this work

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Abstract

A series of [2-(nitroaryl)ethenyl]pyridinium and quinolinium derivatives have been synthesised as potential indicators of microbial nitroreductase activity. When assessed against a selection of 20 clinically important pathogenic microorganisms, microbial colonies of various colours (yellow, green, red, brown, black) were produced and attributed to nitroreductase activity. Most substrates elicited colour responses with Gram-negative microorganisms. In contrast, the growth of several species of Gram-positive microorganisms and yeasts was often inhibited by the substrates and hence coloured responses were not seen.
Original languageEnglish
Pages (from-to)6106-6113
Number of pages8
JournalJournal of Materials Chemistry B
Volume11
Issue number26
Early online date7 Jun 2023
DOIs
Publication statusPublished - 14 Jul 2023

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