Cigarette smoking induced decrease in fibronectin and COL1A1 in locomotor muscle in a guinea pig COPD model

Efpraxia Kritikaki, Victor Peinado, Joao Barbera, Esther Barreiro, Kay Padget, Davina C M Simoes*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

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Abstract

Background: Sarcopenia is a major comorbidity in patients with chronic obstructive pulmonary disease (COPD) caused by decreased physical activity capacity, cigarette smoke (CS) and chronic hypoxia (CH). These risk factors affect muscle anabolic and catabolic biomarkers responsible for sarcopenia. However, whether CS and CH affect muscle extracellular matrix (ECM) biomarkers is still unknown. Intramuscular ECM plays an important role in muscle growth and repair processes, but their role in sarcopenia is under investigated. The aim of this study was to investigate alterations in the expression of intramuscular ECMs biomarkers in response to CH and CS. We hypothesize that altered expression of the ECM molecules promoted by CH and CS contribute to development of sarcopenia.
Methods: Skeletal muscle from a guinea pig animal model exposed (a) to 12 weeks CS (CS group), (b) to 10-week normal atmospheric air followed by 2-week of inspiratory oxygen fraction (FI,O2) of 0.12 in a hypoxic chamber (CH group), (c) to 12-week CS followed by 2-week CH (CS-CH group), (d) to 12-week sham CS followed by 2-week normal atmospheric air (Control group) were analysed by Real-time PCR for the change in expression of intramuscular ECM biomarkers.
Results: Only CS treatment induced significant decreased in fibronectin and COLA1 transcriptomes by 43% (p=0.05) and by 84.1% (p=0.026) respectively, compared to controls. CH and CS-CH treatment downregulated fibronectin and COLA1, but not significantly compared to controls. No significant differences in ECM gene expression were observed when comparing CH with CS treatment.
Conclusion: CS affects ECM composition altering the expression of fibronectin and COL1A1. The role of ECM biomarkers to muscle sarcopenia is being investigated.
Original languageEnglish
Title of host publicationProceedings 2021 International Sarcopenia Translational Research Conference
Subtitle of host publicationCommunications to the International Sarcopenia Translational Research Conference 2021
Place of PublicationAthens, Greece
PublisherHylonome Publications
Pages173-174
Number of pages2
Volume6
Edition3
DOIs
Publication statusPublished - 1 Sep 2021
EventInternational Sarcopenia Translational Research Conference 2021: Where worlds meet - Virtual, Newcastle University, Newcastle Upon Tyne, United Kingdom
Duration: 10 Jun 202111 Jun 2021
https://conferences.ncl.ac.uk/istrc20/

Publication series

NameJournal of Frailty Sarcopenia & Falls
PublisherHylonome Publications
Number3
Volume6
ISSN (Print)2459-4148

Conference

ConferenceInternational Sarcopenia Translational Research Conference 2021
Country/TerritoryUnited Kingdom
CityNewcastle Upon Tyne
Period10/06/2111/06/21
Internet address

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