Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis

Edward Taylor, Simon Charnock, John Colby, Gideon Davies, Gary Black

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3 Citations (Scopus)

Abstract

The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 Å using an in-house Cu Kα source and belong to either space group P6522 or P6122, with unit-cell parameters a = b = 72.4, c = 241.2 Å, and have two molecules of YckF in the asymmetric unit.
Original languageEnglish
Pages (from-to)1138-1140
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number8
DOIs
Publication statusPublished - 2001

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