Co-immobilization multienzyme nanoreactor with co-factor regeneration for conversion of CO2

Sizhu Ren, Ziyuan Wang, Muhammad Bilal, Yuxiao Feng, Yunhong Jiang, Shiru Jia, Jiandong Cui

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Multienzymatic conversion of carbon dioxide (CO 2) into chemicals has been extensively studied. However, regeneration and reuse of co-factor are still the main problems for the efficient conversion of CO 2. In this study, a nanoscale multienzyme reactor was constructed by encapsulating simultaneously carbonic anhydrase (CA), formate dehydrogenase (FateDH), co-factor (NADH), and glutamate dehydrogenases (GDH) into ZIF-8. In the multienzyme reactors, cationic polyelectrolyte (polyethyleneimine, PEI) was doped in the ZIF-8 by dissolving it in the precursors of ZIF-8. Co-factor (NADH) was anchored in ZIF-8 by ion exchange between PEI (positive charge) and co-factor (negative charge), and regenerated through GDH embedded in the ZIF-8, thus keeping high activity of FateDH. Activity recovery of FateDH in the multienzyme reactors reached 50%. Furthermore, the dissolution of CO 2 in the reaction solution was increased significantly by the combination of CA and ZIF-8. As a result, the nanoscale multienzyme reactor exhibited superior capacity for conversion of CO 2 to formate. Compared with free multienzyme system, formate yield was increased 4.6-fold by using the nanoscale multienzyme reactor. Furthermore, the nanoscale multienzyme reactor still retained 50% of its original productivity after 8 cycles, indicating excellent reusability.

Original languageEnglish
Pages (from-to)110-118
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume155
Early online date24 Mar 2020
DOIs
Publication statusPublished - 15 Jul 2020

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