@article{26df449b682e4d3d8212220f59a0f4fd,
title = "Deriving a continuum score for group 3 and 4 medulloblastoma tumor samples analyzed via RNA-sequencing or DNA methylation microarray",
abstract = "Here, we present a protocol for deriving a continuum score for group 3 and 4 medulloblastoma tumor samples analyzed via RNA-sequencing or DNA methylation microarray. We describe steps for utilizing NMF-defined group 3/group 4 metagenes to calculate a continuum score between 0 and 1 that can be projected onto new sample data analyzed via RNA-sequencing. We then detail procedures for reverse engineering a continuum score for samples analyzed via DNA methylation microarray using a random forest classifier.",
keywords = "Bioinformatics, Cancer, Molecular Biology, RNAseq, Sequence analysis, Sequencing",
author = "Hacking, {James P.R.} and Dean Thompson and Schwalbe, {Edward C.} and Clifford, {Steven C.} and Daniel Williamson",
note = "Funding information: This work was supported by Cancer Research UK (C8464/A13457 and C8464/A23391), the Tom Graham Trust/CCLG, LoveOliver, Star for Harris and the INSTINCT network, co-funded by The Brain Tumour Charity, Great Ormond Street Children{\textquoteright}s Charity, and Children with Cancer UK (grant no. 16/193).",
year = "2023",
month = sep,
day = "15",
doi = "10.1016/j.xpro.2023.102509",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",
}
