Detection of carboxypeptidases as taxonomic markers in gram-negative bacteria.

John Perry, L. Archer, Amanda Jones, Arthur James, Michael Ford, Frances Kate Gould

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The use of seven benzoyl- L-amino acids for the detection of carboxypeptidase activities in strains of Gram-negative aerobic and facultatively anaerobic bacteria is reported. A simple overnight assay was designed using ninhydrin for the demonstration of the amino acid released by hydrolysis. Detection of carboxypeptidase activity was shown to have some taxonomic relevance within the Enterobacteriaceae; it was also useful for differentiation within the genus Acinetobacter and for distinction between Pseudomonas aeruginosa and Pseudomonas fluorescens.
Original languageEnglish
Pages (from-to)359-362
JournalLetters in Applied Microbiology
Issue number5
Publication statusPublished - May 1998


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