Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates

Marie Cellier; Arthur James; Sylvain Orenga; John Perry; Ari Rasul; Stephen Stanforth

doi:10.1016/j.bmcl.2017.03.080

Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates

Marie Cellier, Arthur James, Sylvain Orenga, John Perry, Ari Rasul, Stephen Stanforth

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Abstract

Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detecting L-alanylaminopeptidase activity in microorganisms. An Alizarin-based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions.

Original language	English
Pages (from-to)	2102-2106
Journal	Bioorganic & Medicinal Chemistry Letters
Volume	27
Issue number	10
Early online date	28 Mar 2017
DOIs	https://doi.org/10.1016/j.bmcl.2017.03.080
Publication status	Published - 15 May 2017

Keywords

Chromogenic enzyme substrates
L-alanylaminopeptidase detection
Microorganisms

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10.1016/j.bmcl.2017.03.080

Cellier et al - Chromogenic spacers accepted versionAccepted author manuscript, 544 KB
Cellier et al - Chromogenic spacers graphical abstractOther version, 15.8 KB

http://nrl.northumbria.ac.uk/30282/Licence: CC BY-NC-ND

Cite this

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title = "Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates",

abstract = "Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detecting L-alanylaminopeptidase activity in microorganisms. An Alizarin-based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions.",

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AU - Orenga, Sylvain

AU - Perry, John

AU - Rasul, Ari

AU - Stanforth, Stephen

PY - 2017/5/15

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KW - Chromogenic enzyme substrates

KW - L-alanylaminopeptidase detection

KW - Microorganisms

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Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates

Abstract

Keywords

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