Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

Marie Cellier, Arthur James, Jonathan Lowe, Sylvain Orenga, John Perry, Ari Rasul, Stephen Stanforth

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
5 Downloads (Pure)

Abstract

A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.
Original languageEnglish
Pages (from-to)4066-4074
JournalBioorganic & Medicinal Chemistry
Volume24
Issue number18
Early online date27 Jun 2016
DOIs
Publication statusPublished - 15 Sep 2016

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