Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

Marie Cellier; Arthur James; Jonathan Lowe; Sylvain Orenga; John Perry; Ari Rasul; Stephen Stanforth

doi:10.1016/j.bmc.2016.06.051

Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

Marie Cellier, Arthur James, Jonathan Lowe, Sylvain Orenga, John Perry, Ari Rasul, Stephen Stanforth

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Abstract

A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.

Original language	English
Pages (from-to)	4066-4074
Journal	Bioorganic & Medicinal Chemistry
Volume	24
Issue number	18
Early online date	27 Jun 2016
DOIs	https://doi.org/10.1016/j.bmc.2016.06.051
Publication status	Published - 15 Sept 2016

Keywords

Fluorogenic enzyme substrates
l-Alanylaminopeptidase detection
Microorganisms

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10.1016/j.bmc.2016.06.051

Stanforth text for NRLAccepted author manuscript, 568 KB

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title = "Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates",

abstract = "A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.",

keywords = "Fluorogenic enzyme substrates, l-Alanylaminopeptidase detection, Microorganisms",

author = "Marie Cellier and Arthur James and Jonathan Lowe and Sylvain Orenga and John Perry and Ari Rasul and Stephen Stanforth",

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AU - Cellier, Marie

AU - James, Arthur

AU - Lowe, Jonathan

AU - Orenga, Sylvain

AU - Perry, John

AU - Rasul, Ari

AU - Stanforth, Stephen

PY - 2016/9/15

Y1 - 2016/9/15

N2 - A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.

AB - A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.

KW - Fluorogenic enzyme substrates

KW - l-Alanylaminopeptidase detection

KW - Microorganisms

U2 - 10.1016/j.bmc.2016.06.051

DO - 10.1016/j.bmc.2016.06.051

M3 - Article

SN - 0968-0896

SN - 1464-3391

VL - 24

SP - 4066

EP - 4074

JO - Bioorganic & Medicinal Chemistry

JF - Bioorganic & Medicinal Chemistry

IS - 18

ER -

Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

Abstract

Keywords

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