Development and Characterisation of Acoustofluidic Devices Using Detachable Electrodes Made from PCB

Roman Mikhaylov; Fangda Wu; Hanlin Wang; Aled Clayton; Chao Sun; Zhihua Xie; Dongfang Liang; Yinhua Dong; Fan Yuan; Despina Moschou; Zhenlin Wu; Ming Hong Shen; Jian Yang; Richard Fu; Zhiyong Yang; Christian Burton; Rachel Errington; Marie Wiltshire; Xin Yang

doi:10.1039/C9LC01192G

Development and Characterisation of Acoustofluidic Devices Using Detachable Electrodes Made from PCB

Roman Mikhaylov, Fangda Wu, Hanlin Wang, Aled Clayton, Chao Sun, Zhihua Xie, Dongfang Liang, Yinhua Dong, Fan Yuan, Despina Moschou, Zhenlin Wu, Ming Hong Shen, Jian Yang, Richard Fu, Zhiyong Yang, Christian Burton, Rachel Errington, Marie Wiltshire, Xin Yang

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Abstract

Acoustofluidics has been increasingly applied in biology, medicine and chemistry due to its versatility in manipulating fluids, cells and nano-/micro-particles. In this paper, we develop a novel and simple technology to fabricate a surface acoustic wave (SAW)-based acoustofluidic device by clamping electrodes made using a printed circuit board (PCB) with a piezoelectric substrate. The PCB-based SAW (PCB-SAW) device is systematically characterised and benchmarked with a SAW device made using the conventional photolithography process with the same specifications. Microparticle manipulations such as streaming in droplets and patterning in microchannels were demonstrated in the PCB-SAW device. In addition, the PCB-SAW device was applied as an acoustic tweezer to pattern lung cancer cells to form three or four traces inside the microchannel in a controllable manner. Cell viability of ~97% was achieved after acoustic manipulation using the PCB-SAW device, which proved its ability as a suitable tool for acoustophoretic applications.

Original language	English
Pages (from-to)	1807-1814
Number of pages	8
Journal	Lab on a Chip - Miniaturisation for Chemistry and Biology
Volume	20
Issue number	10
Early online date	14 Apr 2020
DOIs	https://doi.org/10.1039/C9LC01192G
Publication status	Published - 21 May 2020

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Mikhaylov, R., Wu, F., Wang, H., Clayton, A., Sun, C., Xie, Z., Liang, D., Dong, Y., Yuan, F., Moschou, D., Wu, Z., Shen, M. H., Yang, J., Fu, R., Yang, Z., Burton, C., Errington, R., Wiltshire, M., & Yang, X. (2020). Development and Characterisation of Acoustofluidic Devices Using Detachable Electrodes Made from PCB. Lab on a Chip - Miniaturisation for Chemistry and Biology, 20(10), 1807-1814. https://doi.org/10.1039/C9LC01192G

@article{be1f765cd22a488eab40a0bc1eb26606,

title = "Development and Characterisation of Acoustofluidic Devices Using Detachable Electrodes Made from PCB",

abstract = "Acoustofluidics has been increasingly applied in biology, medicine and chemistry due to its versatility in manipulating fluids, cells and nano-/micro-particles. In this paper, we develop a novel and simple technology to fabricate a surface acoustic wave (SAW)-based acoustofluidic device by clamping electrodes made using a printed circuit board (PCB) with a piezoelectric substrate. The PCB-based SAW (PCB-SAW) device is systematically characterised and benchmarked with a SAW device made using the conventional photolithography process with the same specifications. Microparticle manipulations such as streaming in droplets and patterning in microchannels were demonstrated in the PCB-SAW device. In addition, the PCB-SAW device was applied as an acoustic tweezer to pattern lung cancer cells to form three or four traces inside the microchannel in a controllable manner. Cell viability of \textasciitilde{}97\% was achieved after acoustic manipulation using the PCB-SAW device, which proved its ability as a suitable tool for acoustophoretic applications. ",

author = "Roman Mikhaylov and Fangda Wu and Hanlin Wang and Aled Clayton and Chao Sun and Zhihua Xie and Dongfang Liang and Yinhua Dong and Fan Yuan and Despina Moschou and Zhenlin Wu and Shen, \{Ming Hong\} and Jian Yang and Richard Fu and Zhiyong Yang and Christian Burton and Rachel Errington and Marie Wiltshire and Xin Yang",

note = "The authors would gratefully acknowledge the financial support from EPSRC (EP/P002803/1, EP/P018998/1), EPSRC IAA, Welcome Trust, Global Challenges Research Fund (GCRF), the Royal Society (IEC\textbackslash{}NSFC\textbackslash{}170142, IE161019), and the Natural Science Foundation of China (NSFC) (Grant No. 51811530310).",

year = "2020",

month = may,

day = "21",

doi = "10.1039/C9LC01192G",

language = "English",

volume = "20",

pages = "1807--1814",

journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",

issn = "1473-0197",

publisher = "Royal Society of Chemistry",

number = "10",

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Mikhaylov, R, Wu, F, Wang, H, Clayton, A, Sun, C, Xie, Z, Liang, D, Dong, Y, Yuan, F, Moschou, D, Wu, Z, Shen, MH, Yang, J, Fu, R, Yang, Z, Burton, C, Errington, R, Wiltshire, M & Yang, X 2020, 'Development and Characterisation of Acoustofluidic Devices Using Detachable Electrodes Made from PCB', Lab on a Chip - Miniaturisation for Chemistry and Biology, vol. 20, no. 10, pp. 1807-1814. https://doi.org/10.1039/C9LC01192G

TY - JOUR

T1 - Development and Characterisation of Acoustofluidic Devices Using Detachable Electrodes Made from PCB

AU - Mikhaylov, Roman

AU - Wu, Fangda

AU - Wang, Hanlin

AU - Clayton, Aled

AU - Sun, Chao

AU - Xie, Zhihua

AU - Liang, Dongfang

AU - Dong, Yinhua

AU - Yuan, Fan

AU - Moschou, Despina

AU - Wu, Zhenlin

AU - Shen, Ming Hong

AU - Yang, Jian

AU - Fu, Richard

AU - Yang, Zhiyong

AU - Burton, Christian

AU - Errington, Rachel

AU - Wiltshire, Marie

AU - Yang, Xin

N1 - The authors would gratefully acknowledge the financial support from EPSRC (EP/P002803/1, EP/P018998/1), EPSRC IAA, Welcome Trust, Global Challenges Research Fund (GCRF), the Royal Society (IEC\NSFC\170142, IE161019), and the Natural Science Foundation of China (NSFC) (Grant No. 51811530310).

PY - 2020/5/21

Y1 - 2020/5/21

N2 - Acoustofluidics has been increasingly applied in biology, medicine and chemistry due to its versatility in manipulating fluids, cells and nano-/micro-particles. In this paper, we develop a novel and simple technology to fabricate a surface acoustic wave (SAW)-based acoustofluidic device by clamping electrodes made using a printed circuit board (PCB) with a piezoelectric substrate. The PCB-based SAW (PCB-SAW) device is systematically characterised and benchmarked with a SAW device made using the conventional photolithography process with the same specifications. Microparticle manipulations such as streaming in droplets and patterning in microchannels were demonstrated in the PCB-SAW device. In addition, the PCB-SAW device was applied as an acoustic tweezer to pattern lung cancer cells to form three or four traces inside the microchannel in a controllable manner. Cell viability of ~97% was achieved after acoustic manipulation using the PCB-SAW device, which proved its ability as a suitable tool for acoustophoretic applications.

AB - Acoustofluidics has been increasingly applied in biology, medicine and chemistry due to its versatility in manipulating fluids, cells and nano-/micro-particles. In this paper, we develop a novel and simple technology to fabricate a surface acoustic wave (SAW)-based acoustofluidic device by clamping electrodes made using a printed circuit board (PCB) with a piezoelectric substrate. The PCB-based SAW (PCB-SAW) device is systematically characterised and benchmarked with a SAW device made using the conventional photolithography process with the same specifications. Microparticle manipulations such as streaming in droplets and patterning in microchannels were demonstrated in the PCB-SAW device. In addition, the PCB-SAW device was applied as an acoustic tweezer to pattern lung cancer cells to form three or four traces inside the microchannel in a controllable manner. Cell viability of ~97% was achieved after acoustic manipulation using the PCB-SAW device, which proved its ability as a suitable tool for acoustophoretic applications.

UR - https://www.scopus.com/pages/publications/85084941107

U2 - 10.1039/C9LC01192G

DO - 10.1039/C9LC01192G

M3 - Article

SN - 1473-0197

VL - 20

SP - 1807

EP - 1814

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

IS - 10

ER -

Development and Characterisation of Acoustofluidic Devices Using Detachable Electrodes Made from PCB

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