TY - JOUR
T1 - Development of a method to measure laccase activity on methoxyphenolic food ingredients and isomers
AU - Manzano-Nicolas, Jesus
AU - Marin-Iniesta, Fulgencio
AU - Taboada-Rodriguez, Amaury
AU - Garcia-Canovas, Francisco
AU - Tudela-Serrano, Jose
AU - Muñoz-Muñoz, Jose Luis
N1 - Funding information: This work was partially supported by several grants, FEDER RTC-2017-5964-2 InsectFlour project “Aprovechamiento de subproductos industriales agrícolas para la producción de harinas de insectos para consumo humano y animal”/“Exploitation of industrial agronomic by-products to the production of insect flours for human and animal consumption” from Ministerio de Ciencia, Innovacion y Universidades (Madrid, Spain), 20961/PI/18 project from Fundacion Seneca (CARM, Murcia, Spain), and AEIP-15452 project from Murcia University (Murcia, Spain). JMN has a research contract from RTC-2017-5964-2 project.
PY - 2020/5/15
Y1 - 2020/5/15
N2 - We studied the laccase-catalysed oxygenation of methoxyphenolic food ingredients, such as 2-methoxyphenol (guaiacol) and 2,6-dimethoxyphenol (syringol), isomers such as 3- and 4-methoxyphenol, and 2,3-, 3,4- and 3,5-dimethoxyphenol. These methoxyphenolic substrates generate unstable free radicals, which leads to the erroneous determination of steady state rates. The addition of small quantities of ascorbic acid as coupling reagent generates a lag period because it reduces free radicals to methoxyphenols. Measurement of the length of the lag period provides the reliable determination of true steady state rates. We describe the application of this chronometric method to the kinetic characterization of the oxidation of the above methoxyphenolic substrates by Trametes versicolor laccase.
AB - We studied the laccase-catalysed oxygenation of methoxyphenolic food ingredients, such as 2-methoxyphenol (guaiacol) and 2,6-dimethoxyphenol (syringol), isomers such as 3- and 4-methoxyphenol, and 2,3-, 3,4- and 3,5-dimethoxyphenol. These methoxyphenolic substrates generate unstable free radicals, which leads to the erroneous determination of steady state rates. The addition of small quantities of ascorbic acid as coupling reagent generates a lag period because it reduces free radicals to methoxyphenols. Measurement of the length of the lag period provides the reliable determination of true steady state rates. We describe the application of this chronometric method to the kinetic characterization of the oxidation of the above methoxyphenolic substrates by Trametes versicolor laccase.
KW - 2,6-Dimethoxyphenol
KW - Chronometric method
KW - Guaiacol
KW - Laccase
KW - Methoxyphenols
KW - Trametes versicolor
UR - https://www.scopus.com/pages/publications/85076834203
U2 - 10.1016/j.ijbiomac.2019.10.152
DO - 10.1016/j.ijbiomac.2019.10.152
M3 - Article
C2 - 31751732
AN - SCOPUS:85076834203
SN - 0141-8130
VL - 151
SP - 1099
EP - 1107
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -