Development of Specific Oligonucleotide Probes to DetectVibrioSpecies

Umberto Molini, Narut Thanantong, Annunziata Giangaspero, Olivier Sparagano

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.
Original languageEnglish
Pages (from-to)312-314
JournalAnnals of the New York Academy of Sciences
Volume1149
Issue number1
DOIs
Publication statusPublished - 2008

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