Development of Specific Oligonucleotide Probes to DetectVibrioSpecies

Umberto Molini; Narut Thanantong; Annunziata Giangaspero; Olivier Sparagano

doi:10.1196/annals.1428.049

Development of Specific Oligonucleotide Probes to DetectVibrioSpecies

Umberto Molini, Narut Thanantong, Annunziata Giangaspero, Olivier Sparagano

Northumbria University

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

Original language	English
Pages (from-to)	312-314
Journal	Annals of the New York Academy of Sciences
Volume	1149
Issue number	1
DOIs	https://doi.org/10.1196/annals.1428.049
Publication status	Published - 2008

Keywords

vibrio
reverse line blot
PCR
diagnostic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1196/annals.1428.049

Cite this

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abstract = "Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.",

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T1 - Development of Specific Oligonucleotide Probes to DetectVibrioSpecies

AU - Molini, Umberto

AU - Thanantong, Narut

AU - Giangaspero, Annunziata

AU - Sparagano, Olivier

PY - 2008

Y1 - 2008

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AB - Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

KW - vibrio

KW - reverse line blot

KW - PCR

KW - diagnostic

U2 - 10.1196/annals.1428.049

DO - 10.1196/annals.1428.049

M3 - Article

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VL - 1149

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EP - 314

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

IS - 1

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Development of Specific Oligonucleotide Probes to DetectVibrioSpecies

Abstract

Keywords

UN SDGs

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