TY - JOUR
T1 - Difference in PNA label intensity between short- and middle-wavelength sensitive cones in the ground squirrel retina
AU - Szél, Agoston
AU - von Schantz, Malcolm
AU - Röhlich, Pál
AU - Farber, Debora B.
AU - van Veen, Theo
PY - 1993/12/1
Y1 - 1993/12/1
N2 - Purpose. Peanut agglutinin lectin (PNA) is known for its selective binding to cone cells and to the cone domains of the interphotoreceptor matrix. In the current study, the authors investigated whether there is any difference in PNA binding between color-specific cones of the cone-dominant ground squirrel. Methods. Consecutive serial sections of the retina of Spermophilus tridecemlineatus were reacted alternately with PNA and antivisual pigment antibodies. The PNA labels associated with short- and middle-wavelength- sensitive cones (S-cones and M-cones, respectively) were compared with fluorescent lectin cytochemistry. Results. Although all rod-like cells were left unstained, the cones exhibited a specific lectin label. There was, however, a significant difference between the two cone types; the intensity of the ring-like PNA label in the matrix sheath around S-cones significantly exceeded that of the M-cones. Conclusions. The difference in PNA label intensity indicates a difference in the composition of the matrix sheaths surrounding the two respective cone types. To the authors' knowledge, this is the first report on lectin-cytochemical discrimination of cone matrix sheaths and the first lectin study in the ground squirrel retina leading to the observation that PNA can distinguish the three characteristic photoreceptor types in this animal. In this respect, the rod-like cells of the ground squirrel retina were shown to be no different from rod cells of other species.
AB - Purpose. Peanut agglutinin lectin (PNA) is known for its selective binding to cone cells and to the cone domains of the interphotoreceptor matrix. In the current study, the authors investigated whether there is any difference in PNA binding between color-specific cones of the cone-dominant ground squirrel. Methods. Consecutive serial sections of the retina of Spermophilus tridecemlineatus were reacted alternately with PNA and antivisual pigment antibodies. The PNA labels associated with short- and middle-wavelength- sensitive cones (S-cones and M-cones, respectively) were compared with fluorescent lectin cytochemistry. Results. Although all rod-like cells were left unstained, the cones exhibited a specific lectin label. There was, however, a significant difference between the two cone types; the intensity of the ring-like PNA label in the matrix sheath around S-cones significantly exceeded that of the M-cones. Conclusions. The difference in PNA label intensity indicates a difference in the composition of the matrix sheaths surrounding the two respective cone types. To the authors' knowledge, this is the first report on lectin-cytochemical discrimination of cone matrix sheaths and the first lectin study in the ground squirrel retina leading to the observation that PNA can distinguish the three characteristic photoreceptor types in this animal. In this respect, the rod-like cells of the ground squirrel retina were shown to be no different from rod cells of other species.
KW - cone matrix sheath
KW - immunocytochemistry
KW - interphotoreceptor matrix
KW - lectin cytochemistry
KW - peanut agglutinin lectin
UR - http://www.scopus.com/inward/record.url?scp=0027770706&partnerID=8YFLogxK
M3 - Article
C2 - 8258523
AN - SCOPUS:0027770706
VL - 34
SP - 3641
EP - 3645
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 13
ER -