TY - JOUR
T1 - Disrupting biofilm and eradicating bacteria by Ag-Fe3O4@ MoS2 MNPs nanocomposite carrying enzyme and antibiotics
AU - Baig, Mirza Muhammad Faran Ashraf
AU - Fatima, Arshia
AU - Gao, Xiuli
AU - Farid, Awais
AU - Ajmal Khan, Muhammad
AU - Zia, Abdul Wasy
AU - Wu, Hongkai
N1 - Funding Information:
The work was supported by Hong Kong Research Grant Council (RGC) funding projects (GRF# 16308818 , GRF# 16309920 , and GRF# 16309421 ), Hong Kong Innovation and Technology Commission (HKITC) funding projects ( MHP/003/19, and ITC-CNERC14SC01 ) and the Social Development Project of Guizhou Department of Science and Technology ([No.2020]4Y214 ).
PY - 2022/12/1
Y1 - 2022/12/1
N2 - In this study, novel multilayered magnetic nanoparticles (ML-MNPs) loaded with DNase and/or vancomycin (Vanc) were fabricated for eliminating multispecies biofilms. Iron-oxide MNPs (IO-core) (500-800 nm) were synthesized via co-precipitation; further, the IO-core was coated with heavy-metal-based layers (Ag and MoS NPs) using solvent evaporation. DNase and Vanc were loaded onto the outermost layer of the ML-MNP formed by nanoporous MoS NPs through physical deposition and adsorption. The biofilms of S. mutans or E. faecalis (or both) were formed in a brain-heart-infusion broth (BHI) for 3 days, followed by treatment with ML-MNPs for 24 h. The results revealed that coatings of Ag (200 nm) and ultrasmall MoS (20 nm) were assembled as outer layers of ML-MNPs successfully, and they formed Ag-FeO@MoS MNPs (3-5 μm). The DNase-Vanc-loaded MNPs caused nanochannels digging and resulted in the enhanced penetration of MNPs towards the bottom layers of biofilm, which resulted in a decrease in the thickness of the 72-h biofilm from 48 to 58 μm to 0-4 μm. The sustained release of Vanc caused a synergistic bacterial killing up to 96%-100%. The heavy-metal-based layers of MNPs act as nanozymes to interfere with bacterial metabolism and proliferation, which adversely affects biofilm integrity. Further, loading DNase/Vanc onto the nanoporous-MoS -layer of ML-MNPs promoted nanochannel creation through the biofilm. Therefore, DNase-and Vanc-loaded ML-MNPs exhibited potent effects on biofilm disruption and bacterial killing. [Abstract copyright: Copyright © 2022 Elsevier B.V. All rights reserved.]
AB - In this study, novel multilayered magnetic nanoparticles (ML-MNPs) loaded with DNase and/or vancomycin (Vanc) were fabricated for eliminating multispecies biofilms. Iron-oxide MNPs (IO-core) (500-800 nm) were synthesized via co-precipitation; further, the IO-core was coated with heavy-metal-based layers (Ag and MoS NPs) using solvent evaporation. DNase and Vanc were loaded onto the outermost layer of the ML-MNP formed by nanoporous MoS NPs through physical deposition and adsorption. The biofilms of S. mutans or E. faecalis (or both) were formed in a brain-heart-infusion broth (BHI) for 3 days, followed by treatment with ML-MNPs for 24 h. The results revealed that coatings of Ag (200 nm) and ultrasmall MoS (20 nm) were assembled as outer layers of ML-MNPs successfully, and they formed Ag-FeO@MoS MNPs (3-5 μm). The DNase-Vanc-loaded MNPs caused nanochannels digging and resulted in the enhanced penetration of MNPs towards the bottom layers of biofilm, which resulted in a decrease in the thickness of the 72-h biofilm from 48 to 58 μm to 0-4 μm. The sustained release of Vanc caused a synergistic bacterial killing up to 96%-100%. The heavy-metal-based layers of MNPs act as nanozymes to interfere with bacterial metabolism and proliferation, which adversely affects biofilm integrity. Further, loading DNase/Vanc onto the nanoporous-MoS -layer of ML-MNPs promoted nanochannel creation through the biofilm. Therefore, DNase-and Vanc-loaded ML-MNPs exhibited potent effects on biofilm disruption and bacterial killing. [Abstract copyright: Copyright © 2022 Elsevier B.V. All rights reserved.]
KW - Bactericidal effects
KW - Biofilm eradication
KW - DNase/Vancomycin loading
KW - Iron-oxide core
KW - Multilayered magnetic nanoparticles
KW - Nanochannel digging
KW - Silver/MoS nanozyme layers
UR - http://www.scopus.com/inward/record.url?scp=85140142259&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2022.10.009
DO - 10.1016/j.jconrel.2022.10.009
M3 - Article
C2 - 36243235
SN - 0168-3659
VL - 352
SP - 98
EP - 120
JO - Journal of Controlled Release
JF - Journal of Controlled Release
ER -