Draft genomes of twelve host adapted and environmental isolates of Pseudomonas aeruginosa and their position in the core genome phylogeny.

Lewis Stewart, Amy Ford, Vartul Sangal, Julie Jeukens, Brian Boyle, Shabhonam Caim, Lisa Crossman, Paul Hoskisson, Roger Levesque, Nicholas Tucker

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen particularly associated with the inherited disease cystic fibrosis (CF). P. aeruginosa is well known to have a large and adaptable genome that enables it to colonise a wide range of ecological niches. Here we have used a comparative genomics approach to identify changes that occur during infection of the CF lung. We used the mucoid phenotype as an obvious marker of host adaptation and compared these genomes in order to analyse SNPs, indels and islands within near-isogenic pairs. In order to commence the correction of the natural bias towards clinical isolates in genomics studies and to widen our understanding of the genomic diversity of P. aeruginosa, we included four environmental isolates in our analysis. Our data suggests that genome plasticity plays an important role in chronic infection and that the strains sequenced in this study are representative of the two major phylogenetic groups as determined by core genome SNP analysis.
Original languageEnglish
Pages (from-to)20-25
JournalPathogens and Disease
Volume71
Issue number1
DOIs
Publication statusPublished - 26 Jun 2014

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