TY - JOUR
T1 - Dual-Wave Acoustofluidic Centrifuge for Ultrafast Concentration of Nanoparticles and Extracellular Vesicles
AU - Dumcius, Povilas
AU - Mikhaylov, Roman
AU - Zhang, Xiaoyan
AU - Bareford, Matthew
AU - Stringer Martin, Mercedes
AU - Errington, Rachel J.
AU - Sun, Chao
AU - Gonzalez, Esperanza
AU - Krukovski, Tomaš
AU - Falcon-Perez, Juan M.
AU - Liang, Dongfang
AU - Fu, Yongqing (Richard)
AU - Clayton, Aled
AU - Yang, Xin
PY - 2023/8/29
Y1 - 2023/8/29
N2 - Extracellular vesicles (EVs) are secreted nanostructures that play various roles in critical cancer processes. They operate as an intercellular communication system, transferring complex sets of biomolecules from cell to cell. The concentration of EVs is difficult to decipher, and there is an unmet technological need for improved (faster, simpler, and gentler) approaches to isolate EVs from complex matrices. Herein, an acoustofluidic concentration of extracellular vesicles (ACEV) is presented, based on a thin-film printed circuit board with interdigital electrodes mounted on a piezoelectric substrate. An angle of 120° is identified between the electrodes and the reference flat of the piezoelectric substrate for simultaneous generation of Rayleigh and shear horizontal waves. The dual waves create a complex acoustic field in a droplet, resulting in effective concentration of nanoparticles and EVs. The ACEV is able to concentrate 20 nm nanospheres within 105 s and four EV dilutions derived from the human prostate cancer (Du145) cell line in approximately 30 s. Cryo-electron microscopy confirmed the preservation of EV integrity. The ACEV device holds great potential to revolutionize investigations of EVs. Its faster, simpler, and gentler approach to EV isolation and concentration can save time and effort in phenotypic and functional studies of EVs.
AB - Extracellular vesicles (EVs) are secreted nanostructures that play various roles in critical cancer processes. They operate as an intercellular communication system, transferring complex sets of biomolecules from cell to cell. The concentration of EVs is difficult to decipher, and there is an unmet technological need for improved (faster, simpler, and gentler) approaches to isolate EVs from complex matrices. Herein, an acoustofluidic concentration of extracellular vesicles (ACEV) is presented, based on a thin-film printed circuit board with interdigital electrodes mounted on a piezoelectric substrate. An angle of 120° is identified between the electrodes and the reference flat of the piezoelectric substrate for simultaneous generation of Rayleigh and shear horizontal waves. The dual waves create a complex acoustic field in a droplet, resulting in effective concentration of nanoparticles and EVs. The ACEV is able to concentrate 20 nm nanospheres within 105 s and four EV dilutions derived from the human prostate cancer (Du145) cell line in approximately 30 s. Cryo-electron microscopy confirmed the preservation of EV integrity. The ACEV device holds great potential to revolutionize investigations of EVs. Its faster, simpler, and gentler approach to EV isolation and concentration can save time and effort in phenotypic and functional studies of EVs.
KW - extracellular vesicles
KW - nanoparticles
KW - printed circuit boards
KW - surface acoustic waves
UR - http://www.scopus.com/inward/record.url?scp=85153745229&partnerID=8YFLogxK
U2 - 10.1002/smll.202300390
DO - 10.1002/smll.202300390
M3 - Article
AN - SCOPUS:85153745229
SN - 1613-6810
VL - 19
JO - Small
JF - Small
IS - 35
M1 - 2300390
ER -