Background / Objectives: Depending on prior models, especially Technology, Organization and Environment (TOE) framework, this study aims to develop a research model to study critical factors that affect E-Business Adoption in the Banking Sector. Methods / Statistical Analysis: The research model focuses on examining the impact of organizational capabilities, technology readiness, competition intensity and perceived benefits on adoption of e-business. Data gathered from 106 executives in Jordanian banks was employed to test the effects between the research model constructs using a multiple linear regression test. Findings: From the model factors of this study, the results highlight that perceived benefits, competition intensity and technology readiness are significant factors that shape and affect the e-business adoption. Implications: For practice and research are discussed.