Effect of detergent on "promiscuous" inhibitors

Ali J. Ryan, Norman M. Gray, Peter N. Lowe, Chun Wa Chung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

204 Citations (Scopus)

Abstract

The term "promiscuous" inhibitors has been coined for compounds whose inhibition mechanism involves the interaction of aggregates of many compound molecules with the target protein, rather than the binding of individual molecules. This paper demonstrates that promiscuous inhibitors can be differentiated from classical 1:1 inhibitors by the judicious use of detergents, making it possible to configure assays that significantly reduce this undesirable mechanism of inhibition without compromising assay performance.

Original languageEnglish
Pages (from-to)3448-3451
Number of pages4
JournalJournal of Medicinal Chemistry
Volume46
Issue number16
DOIs
Publication statusPublished - 31 Jul 2003
Externally publishedYes

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