Enzymatic oxidation of oleuropein and 3-hydroxytyrosol by laccase, peroxidase and tyrosinase

Jesus Manzano-Nicolas, Amaury Taboada-Rodriguez, Jose Antonio Teruel-Puche, Fulgencio Marin-Iniesta, Francisco García-Molina, Francisco García‐Cánovas, Jose Tudela‐Serrano, Jose Munoz-Munoz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
61 Downloads (Pure)


The oxidation of oleuropein and 3‐hydroxytyrosol by oxidases laccase, tyrosinase, and peroxidase has been studied. The use of a spectrophotometric method and another spectrophotometric chronometric method has made it possible to determine the kinetic parameters Vmax and KM for each enzyme. The highest binding affinity was shown by laccase. The antioxidant capacities of these two molecules have been characterized, finding a very similar primary antioxidant capacity between them. Docking studies revealed the optimal binding position, which was the same for the two molecules and was a catalytically active position. Practical applications: One of the biggest environmental problems in the food industry comes from olive oil mill wastewater with a quantity of approximately 30 million tons per year worldwide. In addition, olive pomace, the solid residue obtained from the olive oil production, is rich in hydroxytyrosol and oleuropein and the action of enzymatic oxidases can give rise to products in their reactions that can lead to polymerization. This polymerization can have beneficial effects because it can increase the antioxidant capacity with potential application on new functional foods or as feed ingredients. Tyrosinase, peroxidase, and laccase are the enzymes degrading these important polyphenols. The application of a spectrophotometric method for laccase and a chronometric method, for tyrosinase and peroxidase, allowed us to obtain the kinetic information of their reactions on hydroxytyrosol and oleuropein. The kinetic information obtained could advance in the understanding of the mechanism of these important industrial enzymes.
Original languageEnglish
Article numbere13803
Pages (from-to)1-14
Number of pages14
JournalJournal of Food Biochemistry
Issue number8
Early online date4 Jul 2021
Publication statusPublished - 1 Aug 2021


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