TY - JOUR
T1 - Evaluation of adjuvanted recombinant urease foreffective immunization againstHelicobacter pylori
AU - Moschos, Sterghios
AU - Somavarapu, S.
AU - Bramwell, Vincent
AU - Randall, L.
AU - Guy, B.
AU - Alpar, Oya
PY - 2001/12
Y1 - 2001/12
N2 - Urease is one of the key enzymes involved in the establishment of infection by the stomach-colonizing pathogen Helicobacter pylori. There combinant enzyme was co-administered intranasally with a range of adjuvants and their combinations. Over 6 months, we measured a number of immunological parameters to characterize both the intensity and quality of the ensuing immune responses. From the formulations tested, chitosan and alum-based adjuvants both elicited strong and sustained humor alurease-specific immunity, with the CTB þ MDP formulation shown to be superior. Following analysis of splenocyte proliferation, marked differences were seen in the concentrations of IL-2, IL-4, IL-6 and IFN-g in the supernatants of stimulated splenocytes, with profiles corresponding to antibody titre patterns. Compared to CTB, a strong inducer of all assayed cytokines, chitosan and alum adjuvants appeared to down-regulate IL-4induction, with no detectable IFN-g. In combination with CTB, MDP follows the same cytokine profile, albeit at rather lower levels, except forIL-2. We conclude that the CTB þ MDP combination offers significant potential with respect to urease-specific CD4þ cell induction, a process recently identified as essential for the clearance of H. pylori.
AB - Urease is one of the key enzymes involved in the establishment of infection by the stomach-colonizing pathogen Helicobacter pylori. There combinant enzyme was co-administered intranasally with a range of adjuvants and their combinations. Over 6 months, we measured a number of immunological parameters to characterize both the intensity and quality of the ensuing immune responses. From the formulations tested, chitosan and alum-based adjuvants both elicited strong and sustained humor alurease-specific immunity, with the CTB þ MDP formulation shown to be superior. Following analysis of splenocyte proliferation, marked differences were seen in the concentrations of IL-2, IL-4, IL-6 and IFN-g in the supernatants of stimulated splenocytes, with profiles corresponding to antibody titre patterns. Compared to CTB, a strong inducer of all assayed cytokines, chitosan and alum adjuvants appeared to down-regulate IL-4induction, with no detectable IFN-g. In combination with CTB, MDP follows the same cytokine profile, albeit at rather lower levels, except forIL-2. We conclude that the CTB þ MDP combination offers significant potential with respect to urease-specific CD4þ cell induction, a process recently identified as essential for the clearance of H. pylori.
U2 - 10.1046/j.1365-2567.2001.1040s1070.x
DO - 10.1046/j.1365-2567.2001.1040s1070.x
M3 - Article
SN - 0019-2805
VL - 104
SP - 98
JO - Immunology
JF - Immunology
IS - S1
ER -