TY - JOUR
T1 - Expression of the peptide transporter hPepT1 in human colon
T2 - A potential route for colonic protein nitrogen and drug absorption
AU - Ford, Dianne
AU - Howard, Alison
AU - Hirst, Barry H.
N1 - Funding Information:
Acknowledgements The authors thank Dr. M.K. Bennett, Department of Pathology, Freeman Hospital, Newcastle upon Tyne, for the supply of human tissue samples. This work was funded by the Biotechnology and Biological Sciences Research Council.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Substrates of the proton-coupled peptide transporter, hPepT1, include dietary di- and tripeptides plus therapeutically important drugs such as the β-lactam antibiotics and angiotensin-converting enzyme inhibitors. Expression and function of hPepT1 in the small bowel is well established. We have compared levels of hPepT1 mRNA expression in regions of human gut by RT-PCR methods and examined the expression of hPepT1 in normal human colon using an anti-hPepT1 antipeptide antibody, hPepT1 mRNA was expressed in the large intestine, although at lower levels than in the small intestine. Quantitatively, expression in ileum was 4.6-fold greater than in sigmoid colon. Immunoreactive hPepT1 was detected in human colon at lower levels than in ileum. The pattern of expression differed between the two tissues: whilst expression in the ileum was localised to the apical enterocyte membrane along the length of the crypt-villus axis, expression in the colonocyte was detected at the apical membrane towards the luminal surface but predominantly at the basal membrane towards the base of the crypt. We conclude that distal regions of the bowel express hPepT1, which may provide a mechanism for colonic protein-nitrogen absorption and for absorption of therapeutically important peptidomimetic drugs.
AB - Substrates of the proton-coupled peptide transporter, hPepT1, include dietary di- and tripeptides plus therapeutically important drugs such as the β-lactam antibiotics and angiotensin-converting enzyme inhibitors. Expression and function of hPepT1 in the small bowel is well established. We have compared levels of hPepT1 mRNA expression in regions of human gut by RT-PCR methods and examined the expression of hPepT1 in normal human colon using an anti-hPepT1 antipeptide antibody, hPepT1 mRNA was expressed in the large intestine, although at lower levels than in the small intestine. Quantitatively, expression in ileum was 4.6-fold greater than in sigmoid colon. Immunoreactive hPepT1 was detected in human colon at lower levels than in ileum. The pattern of expression differed between the two tissues: whilst expression in the ileum was localised to the apical enterocyte membrane along the length of the crypt-villus axis, expression in the colonocyte was detected at the apical membrane towards the luminal surface but predominantly at the basal membrane towards the base of the crypt. We conclude that distal regions of the bowel express hPepT1, which may provide a mechanism for colonic protein-nitrogen absorption and for absorption of therapeutically important peptidomimetic drugs.
KW - Colon
KW - Peptide transporter
KW - Peptidomimetic drug absorption
UR - http://www.scopus.com/inward/record.url?scp=0037285726&partnerID=8YFLogxK
U2 - 10.1007/s00418-002-0479-y
DO - 10.1007/s00418-002-0479-y
M3 - Article
C2 - 12548404
AN - SCOPUS:0037285726
SN - 0948-6143
VL - 119
SP - 37
EP - 43
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 1
ER -