Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels

Rachel J. Jackson, M. R. B. Binet, Lucy J. Lee, Renli Ma, Alastair Graham, Cameron McLeod, Robert Poole

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Escherichia coli possesses two major systems for inorganic phosphate (Pi) uptake. The Pst system (pstSCAB) is inducible by low phosphate concentrations whereas the low-affinity transporter (pitA) has been described as constitutively expressed. PitA catalyses transport of metal [Mg(II), Ca(II)]–phosphate complexes, and mutations in pitA confer Zn(II) resistance. Here we report that pitA transcription is not constitutive; activity of a single-copy pitA–lacZ transcriptional fusion (monolysogen) was maximal at high extracellular Zn(II) (150 ?M), in the absence of added Pi, and in a well-defined pitA mutant strain. Intracellular zinc levels were unaffected by adding Zn(II) to the medium for both the wild-type and mutant strains. However, in the wild-type strain, Mg levels (per gram of dry biomass) fell by eightfold in cells grown with added Zn(II) and by 20-fold when Zn(II) and Pi were added to cultures. Mutation of pitA reduced the effects of external Zn(II) and phosphate levels on Mg pools, consistent with competition or inhibition by Zn(II) of PitA. The mechanism of pitA regulation by extracellular Zn(II) and Pi is unknown but appears not to involve Fur or other well-characterized regulators.
Original languageEnglish
Pages (from-to)219-224
JournalFEMS Microbiology Letters
Volume289
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint Dive into the research topics of 'Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels'. Together they form a unique fingerprint.

Cite this