Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor

Zhen Tian; Hongzhuan Xuan; Yicun Yao; Shengyu Hao; Zhichao Zhang; Bingyuan Zhang; Jingao Zhang; Liqiang Zhang; Xinzhu Sang; Jinhui Yuan; Gerald Farrell; Qiang Wu

doi:10.1038/s44172-024-00332-y

Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor

Zhen Tian, Hongzhuan Xuan, Yicun Yao, Shengyu Hao, Zhichao Zhang, Bingyuan Zhang, Jingao Zhang, Liqiang Zhang, Xinzhu Sang, Jinhui Yuan^*, Gerald Farrell, Qiang Wu^*

^*Corresponding author for this work

Mathematics, Physics and Electrical Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin’s anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method.

Original language	English
Article number	185
Pages (from-to)	1-8
Number of pages	8
Journal	Communications Engineering
Volume	3
Issue number	1
DOIs	https://doi.org/10.1038/s44172-024-00332-y
Publication status	Published - 26 Dec 2024

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Cite this

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title = "Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor",

abstract = "Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin{\textquoteright}s anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method.",

author = "Zhen Tian and Hongzhuan Xuan and Yicun Yao and Shengyu Hao and Zhichao Zhang and Bingyuan Zhang and Jingao Zhang and Liqiang Zhang and Xinzhu Sang and Jinhui Yuan and Gerald Farrell and Qiang Wu",

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doi = "10.1038/s44172-024-00332-y",

language = "English",

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TY - JOUR

T1 - Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor

AU - Tian, Zhen

AU - Xuan, Hongzhuan

AU - Yao, Yicun

AU - Hao, Shengyu

AU - Zhang, Zhichao

AU - Zhang, Bingyuan

AU - Zhang, Jingao

AU - Zhang, Liqiang

AU - Sang, Xinzhu

AU - Yuan, Jinhui

AU - Farrell, Gerald

AU - Wu, Qiang

PY - 2024/12/26

Y1 - 2024/12/26

N2 - Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin’s anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method.

AB - Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin’s anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method.

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JO - Communications Engineering

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