Recently, Magnesium Oxide (MgO) board has been widely used in LSF wall systems because of its improved acoustic properties, impact resistance, structural strength and serviceability. However, their thermal properties and fire performance have not been fully investigated. Therefore, in this research study thermal properties of two different types of MgO boards available in Australia were measured and their fire performance was investigated using three full-scale fire tests of LSF walls lined with two types of MgO board. Although the tests were conducted on two different types of MgO boards with different configurations, the fire test results gave a fire resistance level (FRL) of 30 min, in which the failure was initiated by integrity of the board with either board cracking or board joint opening. This paper presents the details of the thermal property tests and the three full-scale fire tests, and their results. In addition, the effects of different MgO boards, joint configurations and compounds, noggings, screw fastening techniques and cavity insulation on the fire performance of LSF walls are also presented.