Surface acoustic wave (SAW)-based acoustofluidics shows broad applications in biomedicine and chemistry. Conventional manufacturing process for SAW devices uses photolithography and metal deposition, thus requires accessing cleanroom facilities. This study presents an efficient and versatile technique based on a flexible printed circuit board (FPCB) for developing SAW acoustofluidic devices. By mechanically clamping interdigital electrodes (IDEs) made on the FPCB onto a piezoelectric substrate, SAWs can be effectively generated with an additional matching network. The SAW amplitudes were measured by a laser vibrometer, which increases with the applied input voltage. The FPCB-SAW device has been applied to actuate 10- μm microspheres to form strong streaming vortices inside a droplet, and to drive a sessile droplet for transportation on the substrate surface. The use of the FPCB rather than a rigid printed circuit board (PCB) can help cut down on the overall footprint of the device and save space. The low requirement in assembling the FPCB-SAW device can facilitate versatile acoustofluidic applications by providing fast prototyping devices.